Tumor Microenvironment : Study Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , , , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Cham :
Springer International Publishing : Imprint: Springer,
2016.
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| Edición: | 1st ed. 2016. |
| Colección: | Advances in Experimental Medicine and Biology,
899 |
| Materias: |
Tabla de Contenidos:
- 1.Staining against phospho-H2AX (γ-H2AX) as a marker for DNA damage and genomic instability in cancer tissues and cells
- 2.Measuring DNA replication rates in hypoxic conditions
- 3.Isolation of Proteins on Nascent DNA in hypoxia and reoxygenation conditions
- 4.In vivo interrogation of the hypoxic transcriptome of solid tumors: optimizing hypoxic probe labelling with Laser-Capture Microdissection for isolation of high-quality RNA for deep sequencing analysis
- 5.Gas chromatography coupled to mass spectrometry (GC-MS) to study metabolism in cultured cells
- 6.Probing Cancer Cell Metabolism using NMR Spectroscopy
- 7.Measuring the Impact of Microenvironmental Conditions on Mitochondrial Dehydrogenases Activity in Cultured Cells
- 8.Measuring Autophagy in the Context of Cancer
- 9.Methods for studying autophagy within the tumor microenvironment
- 10.Using 3 Dimensional Culture (Spheroids) as an in vitro Model of Tumour Hypoxia
- 11.Isolation of Glioma Initiating Cells for Biological Study
- 12.Isolation of mouse and human tumor associated macrophages
- 13.Mouse models of brain metastasis for unravelling tumour progression
- 14.Quantification of lung metastases from in vivo mouse models
- 15.A multimodal data analysis approach for targeted drug discovery involving topological data analysis (TDA)
- 16.Efficient protocol for the identification of hypoxic cell radiosensitisers.