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170209s2017 xxu| s |||| 0|eng d |
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|a 9781493967599
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| 024 |
7 |
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|a 10.1007/978-1-4939-6759-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Signal Transduction Immunohistochemistry :
|b Methods and Protocols /
|c edited by Alexander E. Kalyuzhny.
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| 250 |
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|a 2nd ed. 2017.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a X, 290 p. 67 illus., 45 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1554
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| 505 |
0 |
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|a Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications -- Antibodies That Work Again and Again and Again -- Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Methods -- Acquisition and Post-Processing of Immunohistochemical Images -- Using Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Signal Transduction -- EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis -- Preservation of Specific Protein Signaling States using Heat Based Stabilizor System -- Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX using Primary Antibodies Raised in the Same Host Species -- Culture, Transfection and Immunocytochemical Analysis of Primary Macrophages -- Phenotyping CD4+ hTh2 Cells by Flow Cytometry: Simultaneous Detection of Transcription Factors, Secreted Cytokines and Surface Markers -- A Coupled Approach Utilizing Immunohistochemistry and Immunocytochemistry to Visualize Cellular Lipophagy -- Apoptotic Bodies: Selective Detection in Extracellular Vesicles -- Activation and Differentiation of Mesenchymal Stem Cells -- High-Sensitivity IHC Detection of Phosphorylated p27/Kip1 in Human Tissues using Secondary Antibody Conjugated to Polymer-HRP -- Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry -- Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D -- Multiplex Immunohistochemistry for Mapping the Tumor Microenvironment -- Validation of Anti-Mouse PDL-1 Goat Polyclonal Antibody Staining with Mouse PDL-1 In Situ Hybridization on Adjacent Sections of Cell Pellets and Mouse Tumors -- Automated Double In Situ Detection of Mouse LGR5 mRNA and Lysozyme Protein in Examining the Neighboring Cell Types of the Mouse Intestinal Crypt -- Automated High Content Screening of g--H2AX Expression in HeLa Cells.
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| 650 |
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|a Immunology.
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| 650 |
1 |
4 |
|a Immunology.
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| 700 |
1 |
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|a Kalyuzhny, Alexander E.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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