Signal Transduction Immunohistochemistry : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Kalyuzhny, Alexander E. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:2nd ed. 2017.
Colección:Methods in Molecular Biology, 1554
Materias:
Tabla de Contenidos:
  • Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications
  • Antibodies That Work Again and Again and Again
  • Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Methods
  • Acquisition and Post-Processing of Immunohistochemical Images
  • Using Digital Quantification of Stained Tissue Microarrays as a Medium-Throughput, Quantitative Method for Measuring the Kinetics of Signal Transduction
  • EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis
  • Preservation of Specific Protein Signaling States using Heat Based Stabilizor System
  • Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX using Primary Antibodies Raised in the Same Host Species
  • Culture, Transfection and Immunocytochemical Analysis of Primary Macrophages
  • Phenotyping CD4+ hTh2 Cells by Flow Cytometry: Simultaneous Detection of Transcription Factors, Secreted Cytokines and Surface Markers
  • A Coupled Approach Utilizing Immunohistochemistry and Immunocytochemistry to Visualize Cellular Lipophagy
  • Apoptotic Bodies: Selective Detection in Extracellular Vesicles
  • Activation and Differentiation of Mesenchymal Stem Cells
  • High-Sensitivity IHC Detection of Phosphorylated p27/Kip1 in Human Tissues using Secondary Antibody Conjugated to Polymer-HRP
  • Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry
  • Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D
  • Multiplex Immunohistochemistry for Mapping the Tumor Microenvironment
  • Validation of Anti-Mouse PDL-1 Goat Polyclonal Antibody Staining with Mouse PDL-1 In Situ Hybridization on Adjacent Sections of Cell Pellets and Mouse Tumors
  • Automated Double In Situ Detection of Mouse LGR5 mRNA and Lysozyme Protein in Examining the Neighboring Cell Types of the Mouse Intestinal Crypt
  • Automated High Content Screening of g
  • H2AX Expression in HeLa Cells.