Sertoli Cells : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Alves, Marco G. (Editor), Oliveira, Pedro F. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2018.
Edition:1st ed. 2018.
Series:Methods in Molecular Biology, 1748
Subjects:
Table of Contents:
  • Establishment of Primary Culture of Sertoli Cells
  • Evaluation of the Purity of Sertoli Cell Primary Cultures
  • Preparation of Testicular Samples for Histology and Immunohistochemistry
  • Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age
  • Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy
  • Sertoli Cells Preparation for Co-Immunoprecipitation
  • Profiling Signaling Proteins in Sertoli Cells by Co-Immunoprecipitation
  • Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy
  • A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells
  • Assessing Autophagy in Sertoli Cells
  • Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells
  • Assessing Sertoli Cells Metabolic Activity
  • Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy
  • Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs
  • Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice
  • Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of the Actin, and Microtubule-Based Cytoskeletons
  • Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay
  • Computational Methods Involved in Evaluating the Toxicity of the Reproductive Roxicants in Sertoli Cell
  • A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells
  • Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing.