Spliceosomal Pre-mRNA Splicing : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2014.
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| Edition: | 1st ed. 2014. |
| Series: | Methods in Molecular Biology,
1126 |
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Table of Contents:
- The Pre-mRNA Splicing Reaction
- Diversity and Evolution of Spliceosomal Systems
- Mechanisms of Spliceosomal Assembly
- Alternative Pre-mRNA Splicing
- Regulation of Alternative Pre-mRNA Splicing
- Introduction to Co-Transcriptional RNA Splicing
- Chromatin and Splicing
- Preparation of Splicing Competent Nuclear Extracts
- Preparation of Yeast Whole Cell Splicing Extract
- Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase
- In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract
- Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract
- In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation
- Isolation and Accumulation of Spliceosomal Assembly Intermediates
- Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function
- Expression and Purification of Splicing Proteins from Mammalian Cells
- Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis
- Cell-Based Splicing of Minigenes
- Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA
- Antisense Methods to Modulate Pre-mRNA Splicing
- Using Yeast Genetics to Study Splicing Mechanisms
- Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR
- Chromatin Immunoprecipitation Approaches to Determine Co-Transcriptional Nature of Splicing
- Computational Approaches to Mine Publicly Available Databases
- Approaches to Link RNA Secondary Structures with Splicing Regulation
- Methods to Study Splicing from High-Throughput RNA Sequencing Data
- Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq
- Predicting Alternative Splicing.