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03164nam a22003135i 4500 |
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20210611131845.0 |
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cr nn 008mamaa |
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110928s2012 xxu| s |||| 0|eng d |
| 020 |
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|a 9781617793318
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| 024 |
7 |
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|a 10.1007/978-1-61779-331-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Unnatural Amino Acids :
|b Methods and Protocols /
|c edited by Loredano Pollegioni, Stefano Servi.
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| 250 |
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|a 1st ed. 2012.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2012.
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| 300 |
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|a XIV, 409 p. 123 illus. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 794
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| 505 |
0 |
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|a Preparation of Unnatural Amino Acids with Ammonia-lyases and 2,3-aminomutases -- Multistep Enzyme Catalyzed Reactions for Unnatural Amino Acids -- Enzymatic Production of Enantiopure Amino Acids from Mono-substituted Hydantoin Substrates -- Preparation of Glutamate Analogues by Enzymatic Transamination -- Carbon-Carbon Bond Forming Enzymes for the Synthesis of Non-natural Amino Acids.- Engineering Cyclic Amidases for Non-natural Amino Acid Synthesis -- NMR Analysis of Unnatural Amino Acids in Natural Antibiotics -- Site-specific Incorporation of Unnatural Amino Acids as Probes for Protein Conformational Changes -- Application of Unnatural Amino Acids to the De novo Design of Selective Antibiotic Peptides -- Use of Unnatural Amino Acids to Probe Structure-activity Relationships and Mode-of-action of Antimicrobial Peptides -- Experimental Methods for Scanning Unnatural Amino Acid Mutagenesis -- Genetic Incorporation of Unnatural Amino Acids into Proteins in Yeast -- Site-specific Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells -- Incorporation of Unnatural Non-a-amino Acids into the N-terminus of Proteins in a Cell-free Translation System -- Site Specific Modification of Proteins by the Staudinger-phosphite Reaction -- HPLC Methods for Determination of D-aspartate and N-methyl-D-aspartate -- Estimation of Chronological Age from the Racemization Rate of L- and D-aspartic Acid: How to Completely Separate Enantiomers from Dentin -- Enzymatic Detection of D-amino Acids -- An Enzymatic-HPLC Assay to Monitor Endogenous D-serine Release from Neuronal Cultures -- Electrophysiological Analysis of the Modulation of NMDA-receptors Function by d-serine and Glycine in the Central Nervous System -- Biosensors for D-amino Acid Detection -- Analysis of D-β-aspartyl Isomers at Specific Sites in Proteins -- Nutritional Value of D-amino Acids, D-peptides and Amino Acid Derivatives in Mice -- Preparation and Assay of Recombinant Serine Racemase -- Assay of Amino Acid Racemases -- Assays of D-amino Acid Oxidases -- Enzymes Acting on D-amino Acid Containing Peptides.
|
| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Enzymology.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Enzymology.
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| 700 |
1 |
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|a Pollegioni, Loredano.
|e editor.
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| 700 |
1 |
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|a Servi, Stefano.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|