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03062nam a22003255i 4500 |
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20211011141300.0 |
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cr nn 008mamaa |
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130228s2013 xxu| s |||| 0|eng d |
| 020 |
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|a 9781627033114
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| 024 |
7 |
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|a 10.1007/978-1-62703-311-4
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Target Identification and Validation in Drug Discovery :
|b Methods and Protocols /
|c edited by Jurgen Moll, Riccardo Colombo.
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| 250 |
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|a 1st ed. 2013.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a XV, 371 p. 62 illus., 19 illus. in color. :
|b online resource.
|
| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
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| 505 |
0 |
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|a The Path to Oncology Drug Target Validation - an Industry Perspective -- Identification of Aptamers as Specific Binders and Modulators of Cell-Surface Receptor Activity -- The Design and Structure-functional Properties of DNA-based Immunomodulatory Sequences -- siRNA Design Principles and Off-target Effects -- Western Blot Evaluation of siRNA Delivery by pH Responsive Peptides -- High Throughput RNAi Screening for the Identification of Novel Targets -- Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development -- CellProfiler and KNIME: Open Source Tools for High Content Screening -- PARP Inhibition as a Prototype for Synthetic Lethal Screens -- Structure-Based Target Druggability Assessment -- Validating Pharmacological Disruption of Protein-protein Interactions by Acceptor Photobleaching FRET Imaging -- Systematic Analysis of Complex Signal Transduction Pathways using Protein Fragment Complementation Assays -- Reverse-Phase Protein Microarrays and Their Utility in Drug Development -- A Cell Culture System that Mimics Chronic Lymphocytic Leukemia Cells Microenvironment for Drug Screening and Characterization -- 2-Dimensional vs. 3-Dimensional in vitro Tumor Migration and Invasion Assays -- Tumor Spheroid-based Migration Assays for Evaluation of Therapeutic Agents -- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs).-Genetically Engineered Animal Models for in vivo Target Identification and Validation in Oncology -- Target Validation in Mice by Constitutive and Conditional RNAi -- In vivo Target Validation by Inducible RNAi in Human Xenograft Mouse Models -- Brightfield In Situ Hybridization Methods to Discover Gene Amplifications and Rearrangements in Clinical Samples -- Combined microRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers.
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| 650 |
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0 |
|a Pharmacology.
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| 650 |
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0 |
|a Medicinal chemistry.
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| 650 |
1 |
4 |
|a Pharmacology/Toxicology.
|
| 650 |
2 |
4 |
|a Medicinal Chemistry.
|
| 700 |
1 |
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|a Moll, Jurgen.
|e editor.
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| 700 |
1 |
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|a Colombo, Riccardo.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
|
| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|