Target Identification and Validation in Drug Discovery : Methods and Protocols /
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edition: | 1st ed. 2013. |
| Series: | Methods in Molecular Biology,
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| Subjects: |
Table of Contents:
- The Path to Oncology Drug Target Validation - an Industry Perspective
- Identification of Aptamers as Specific Binders and Modulators of Cell-Surface Receptor Activity
- The Design and Structure-functional Properties of DNA-based Immunomodulatory Sequences
- siRNA Design Principles and Off-target Effects
- Western Blot Evaluation of siRNA Delivery by pH Responsive Peptides
- High Throughput RNAi Screening for the Identification of Novel Targets
- Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development
- CellProfiler and KNIME: Open Source Tools for High Content Screening
- PARP Inhibition as a Prototype for Synthetic Lethal Screens
- Structure-Based Target Druggability Assessment
- Validating Pharmacological Disruption of Protein-protein Interactions by Acceptor Photobleaching FRET Imaging
- Systematic Analysis of Complex Signal Transduction Pathways using Protein Fragment Complementation Assays
- Reverse-Phase Protein Microarrays and Their Utility in Drug Development
- A Cell Culture System that Mimics Chronic Lymphocytic Leukemia Cells Microenvironment for Drug Screening and Characterization
- 2-Dimensional vs. 3-Dimensional in vitro Tumor Migration and Invasion Assays
- Tumor Spheroid-based Migration Assays for Evaluation of Therapeutic Agents
- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs).-Genetically Engineered Animal Models for in vivo Target Identification and Validation in Oncology
- Target Validation in Mice by Constitutive and Conditional RNAi
- In vivo Target Validation by Inducible RNAi in Human Xenograft Mouse Models
- Brightfield In Situ Hybridization Methods to Discover Gene Amplifications and Rearrangements in Clinical Samples
- Combined microRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers.