Ribozyme Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1997.
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| Edición: | 1st ed. 1997. |
| Colección: | Methods in Molecular Biology,
74 |
| Materias: |
Tabla de Contenidos:
- Ribozymes
- Computer-Aided Calculation of the Local Folding Potential of Target RNA and Its Use for Ribozyme Design
- Computational Approaches to the Identification of Ribozyme Target Sites
- Computer Analysis of the Conservation and Uniqueness of Ribozyme-Targeted HIV Sequences
- Selection of Accessible Sites for Ribozymes on Large RNA Transcripts
- Selection of Efficient Ribozyme Cleavage Sites in Target RNAs
- Chemical Synthesis, Analysis, and Purification of Ribozymes
- A Practical Method for the Production of RNA and Ribozymes
- Preparation of Templates for Production of Ribozymes and Substrates
- Enzymatic Synthesis and Characterization of Unmodified Ribozymes and Substrates
- T7 Transcript Length Determination Using Enzymatic RNA Sequencing
- Chemical and Enzymatic Approaches to Construct Modified RNAs
- Applications of Modified Transcripts
- Cloning Strategies for Catalytic Antisense RNAs
- PCR-Based Construction of Long Hammerhead Ribozymes
- Design and Production of Asymmetric Hammerhead Ribozymes
- Minimized Hammerhead Ribozymes
- Design of Hairpin Ribozymes for In Vitro and Cellular Applications
- Design of the Hairpin Ribozyme for Targeting Specific RNA Sequences
- Design and Preparation of Sequence-Specific RNase P Ribozymes
- Theoretical Considerations in Measuring Reaction Parameters
- Experimental Approaches for Measuring Reaction Parameters
- Determination of Catalytic Parameters for Hairpin Ribozymes
- Characterizing Ribozyme Cleavage Reactions
- Defining Optimum Reaction Conditions for Hammerhead Ribozymes
- Using Fluorescence Resonance Energy Transfer to Investigate Hammerhead Ribozyme Kinetics
- Design of Hybridizing Arms in Hammerhead Ribozymes
- Optimization of Hammerhead Flanking Sequences Using Oligonucleotide Facilitators
- Enhancement of Ribozyme Function by RNA Binding Proteins
- Selection of Fast-Hybridizing Complementary RNA Species In Vitro
- In Vitro Selection of Hairpin Ribozymes
- The Detection of Hammerhead Ribozyme Cleavage by RT-PCR Methods
- Detection of Ribozyme Cleavage Products Using Reverse Ligation-Mediated PCR (RL-PCR)
- Quantitation of Ribozyme Target Abundance by QCPCR
- Trans-Splicing Reactions by Ribozymes
- Ligation of RNA Molecules by the Hairpin Ribozyme
- Mutagenesis and Modeling of the Hairpin Ribozyme Family
- Preparation of Homogeneous Ribozyme RNA for Crystallization
- Establishing Suitability of RNA Preparations for Crystallization
- A Sparse Matrix Approach to Crystallizing Ribozymes and RNA Motifs
- Crystallographic Analyses of Chemically Synthesized Modified Hammerhead RNA Sequences as a General Approach Toward Understanding Ribozyme Structure and Function
- tRNA Delivery Systems for Ribozymes
- Expressing Ribozymes in Plants
- Using Microinjection of Xenopus Oocytes to Express and Optimize Ribozymes In Vivo
- Exogenous Cellular Delivery of Ribozymes and Ribozyme Encoding DNAs
- Optimization of Lipid-Mediated Ribozyme Delivery to Cells in Culture
- Retroviral Delivery and Anti-HIV Testing of Hammerhead Ribozymes
- Hairpin Ribozyme Gene Therapy for AIDS
- Clinical Aspects of Ribozymes as Therapeutics in Gene Therapy.