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180208s2018 xxu| s |||| 0|eng d |
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|a 9781493975464
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| 024 |
7 |
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|a 10.1007/978-1-4939-7546-4
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Schizosaccharomyces pombe :
|b Methods and Protocols /
|c edited by Teresa L. Singleton.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XII, 244 p. 48 illus., 34 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1721
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| 505 |
0 |
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|a Preparation of Solutions and Reagents -- Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing -- Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe -- Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs -- Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast -- Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast -- Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis -- The No-nonsens SDS-PAGE -- Crystallization of Recombinant α-actinin and Related Proteins -- Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy -- Antibody Pull-down Experiments in Fission Yeast -- Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation -- Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo -- In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S. pombe -- High-frequency Lithium Acetate Transformation of Schizosaccharomyces pomTetrad Dissection in Fission Yeast -- Random Spore Analysis in Fission Yeast -- Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains -- Schizosaccharomyces pombe Biotechnological Applications in Winemaking -- Schizosaccharomyces pombe Isolation Protocol. .
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| 650 |
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|a Microbiology.
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| 650 |
1 |
4 |
|a Microbiology.
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| 700 |
1 |
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|a Singleton, Teresa L.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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