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02258nam a22003375i 4500 |
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000294265 |
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20210616080946.0 |
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cr nn 008mamaa |
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101116s2011 xxu| s |||| 0|eng d |
| 020 |
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|a 9781607619741
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| 024 |
7 |
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|a 10.1007/978-1-60761-974-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Transgenic Mouse Methods and Protocols /
|c edited by Marten H. Hofker, Jan van Deursen.
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| 250 |
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|a 2nd ed. 2011.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2011.
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| 300 |
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|a XI, 352 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 693
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| 505 |
0 |
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|a Introduction: Strategies for developing genetically modified mice -- Genetic Modification of the Mouse; General Technology: Pronuclear and Blastocyst Injection -- Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos -- Cryopreservation of mouse spermatozoa and In vitro fertilization -- Autopsy and histological analysis of the transgenic mouse -- Transgene design -- Inducible Transgenic Mouse Models -- Lentiviral transgenesis -- Transgenesis in Mouse Embryonic Stem Cells -- Engineering BAC Reporter Gene Constructs for Mouse Transgenesis -- Targeting Vector Construction Through Recombineering -- Generating Conditional Knockout Mice -- Hypomorphic mice -- MICER Targeting Vectors for Manipulating the Mouse Genome -- Knockin approaches -- Generation of a series of knockin alleles using RMCE in ES cells -- Selection of targeted mutants from a library of randomly mutagenized ES cells -- Generation of genetically modified rodents using random ENU mutagenesis -- Bone marrow transplantations to study gene function in hematopoietic cells -- Adenovirus Mediated Gene Transfer.
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| 650 |
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0 |
|a Animal genetics.
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| 650 |
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0 |
|a Genetic engineering.
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| 650 |
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0 |
|a Medical genetics.
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| 650 |
1 |
4 |
|a Animal Genetics and Genomics.
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| 650 |
2 |
4 |
|a Genetic Engineering.
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| 650 |
2 |
4 |
|a Gene Function.
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| 700 |
1 |
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|a Hofker, Marten H.
|e editor.
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| 700 |
1 |
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|a van Deursen, Jan.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|