Selenoproteins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Chavatte, Laurent. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1661
Materias:
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245 1 0 |a Selenoproteins :  |b Methods and Protocols /  |c edited by Laurent Chavatte. 
250 |a 1st ed. 2018. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2018. 
300 |a XV, 340 p. 63 illus., 35 illus. in color. :  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 1661 
505 0 |a SECISearch3 and Seblastian: In Silico Tools to Predict SECIS Elements and Selenoproteins -- Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins -- SelGenAmic: An Algorithm for Selenoprotein Gene Assembly -- Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification and Sequencing -- Identification and Characterization of Proteins that Bind to Selenoprotein 3' UTRs -- Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis -- Studying Selenoprotein mRNA Translation using RNA-Seq and Ribosome Profiling -- Modification of Selenoprotein mRNAs by Cap Tri-methylation -- Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) -- Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC – ICP MS) -- Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum -- Radioactive 75Se Labeling and Detection of Selenoproteins -- Non-radioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines -- Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips -- Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues -- Overexpression of Recombinant Selenoproteins in E. coli -- Preparation of Selenocysteine-containing Forms of Human SELENOK and SELENOS -- Selenocysteine Mediated Expressed Protein Ligation of SELENOM -- Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity -- Selective Evaluation of Thioredoxin Reductase Enzymatic Activities -- Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk -- Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis. . 
650 0 |a Proteins . 
650 1 4 |a Protein Science. 
700 1 |a Chavatte, Laurent.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)