Selenoproteins : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edición: | 1st ed. 2018. |
| Colección: | Methods in Molecular Biology,
1661 |
| Materias: |
Tabla de Contenidos:
- SECISearch3 and Seblastian: In Silico Tools to Predict SECIS Elements and Selenoproteins
- Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins
- SelGenAmic: An Algorithm for Selenoprotein Gene Assembly
- Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification and Sequencing
- Identification and Characterization of Proteins that Bind to Selenoprotein 3' UTRs
- Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis
- Studying Selenoprotein mRNA Translation using RNA-Seq and Ribosome Profiling
- Modification of Selenoprotein mRNAs by Cap Tri-methylation
- Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
- Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC – ICP MS)
- Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum
- Radioactive 75Se Labeling and Detection of Selenoproteins
- Non-radioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines
- Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips
- Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues
- Overexpression of Recombinant Selenoproteins in E. coli
- Preparation of Selenocysteine-containing Forms of Human SELENOK and SELENOS
- Selenocysteine Mediated Expressed Protein Ligation of SELENOM
- Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity
- Selective Evaluation of Thioredoxin Reductase Enzymatic Activities
- Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk
- Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis. .