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170114s2017 xxu| s |||| 0|eng d |
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|a 9781493967629
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| 024 |
7 |
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|a 10.1007/978-1-4939-6762-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a SH2 Domains :
|b Methods and Protocols /
|c edited by Kazuya Machida, Bernard A. Liu.
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| 250 |
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|a 1st ed. 2017.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XIV, 558 p. 118 illus., 79 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1555
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| 505 |
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|a Introduction: History of SH2 Domains and Their Applications -- What Have We Learned from SH2 Domains? -- Hidden Markov Models for Protein Domain Homology Identification and Analysis -- Classification and Lineage Tracing of SH2 Domains Throughout Eukaryotes -- SH2 Ligand Prediction-Guidance for In-Silico Screening -- An Efficient Semi-Supervised Learning Approach to Predict SH2 Domain Mediated Interactions -- Proteomic Clustering Analysis of SH2 Domain Datasets -- Expression and Production of SH2 Domain Proteins -- Expression and Purification of Soluble STAT5b/STAT3 Proteins for SH2 Domain Binding Assay -- Purification of SOCS (Suppressors of Cytokine Signalling) SH2 Domains for Structural and Functional Studies -- Expression and Purification of SH2 Domains Using Baculovirus Expression System -- Functionally Altered SH2 Domains for Biochemical Studies: Loss of Function Mutant and Domain Concatenation -- Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain -- Structural Characterization of Monomeric/Dimeric State of p59fyn SH2 Domain -- NMR Chemical Shift Mapping of SH2 Peptide Interactions -- Calorimetric Measurement of SH2 Domain Ligand Affinities -- Binding Assays Using Recombinant SH2 Domains: Far-Western, Pull Down, and Fluorescence Polarization -- In-Solution SH2 Domain Binding Assay Based on Proximity Ligation -- Alpha-Based Multiplexed Assay for Identifying SH2 Domain Antagonists -- Characterizing SH2 Domain Specificity and Network Interactions Using SPOT Peptide Arrays -- High Throughput Quantification of SH2 Domain-Phosphopeptide Interactions with Cellulose Peptide Conjugate Microarrays -- SH2 Domains as Affinity Reagents for Phosphotyrosine Protein Enrichment and Proteomic Analysis -- Identification of Tyrosine Phosphorylated Proteins by SH2 Domain Affinity Purification and Mass Spectrometry -- Analysis of the Global Changes in SH2 Binding Properties Using Mass Spectrometry Supported by Quantitative Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technique -- Using Reciprocal Protein-Peptide Array Screening to Unravel Protein Interaction Networks -- Rosette Assay: Highly Customizable Dot-Blot for SH2 Domain Screening -- Microwestern Arrays for Systems-Level Analysis of SH2 Domain-Containing Proteins -- SH2 Binding Site Protection Assay: A Method for Identification of SH2 Domain Interaction Partners by Exploiting SH2 Mediated Phosphosite Protection -- Real Time Single Molecule Visualization of SH2 Domain Membrane Recruitment in Growth Factor Stimulated Cells -- SH2 Domain-Based FRET Biosensor for Measuring BCR-ABL Activity in Living CML Cells -- SH2 Domain Histochemistry.
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| 650 |
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|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a Machida, Kazuya.
|e editor.
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| 700 |
1 |
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|a Liu, Bernard A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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