SH2 Domains : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edición: | 1st ed. 2017. |
| Colección: | Methods in Molecular Biology,
1555 |
| Materias: |
Tabla de Contenidos:
- Introduction: History of SH2 Domains and Their Applications
- What Have We Learned from SH2 Domains?
- Hidden Markov Models for Protein Domain Homology Identification and Analysis
- Classification and Lineage Tracing of SH2 Domains Throughout Eukaryotes
- SH2 Ligand Prediction-Guidance for In-Silico Screening
- An Efficient Semi-Supervised Learning Approach to Predict SH2 Domain Mediated Interactions
- Proteomic Clustering Analysis of SH2 Domain Datasets
- Expression and Production of SH2 Domain Proteins
- Expression and Purification of Soluble STAT5b/STAT3 Proteins for SH2 Domain Binding Assay
- Purification of SOCS (Suppressors of Cytokine Signalling) SH2 Domains for Structural and Functional Studies
- Expression and Purification of SH2 Domains Using Baculovirus Expression System
- Functionally Altered SH2 Domains for Biochemical Studies: Loss of Function Mutant and Domain Concatenation
- Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain
- Structural Characterization of Monomeric/Dimeric State of p59fyn SH2 Domain
- NMR Chemical Shift Mapping of SH2 Peptide Interactions
- Calorimetric Measurement of SH2 Domain Ligand Affinities
- Binding Assays Using Recombinant SH2 Domains: Far-Western, Pull Down, and Fluorescence Polarization
- In-Solution SH2 Domain Binding Assay Based on Proximity Ligation
- Alpha-Based Multiplexed Assay for Identifying SH2 Domain Antagonists
- Characterizing SH2 Domain Specificity and Network Interactions Using SPOT Peptide Arrays
- High Throughput Quantification of SH2 Domain-Phosphopeptide Interactions with Cellulose Peptide Conjugate Microarrays
- SH2 Domains as Affinity Reagents for Phosphotyrosine Protein Enrichment and Proteomic Analysis
- Identification of Tyrosine Phosphorylated Proteins by SH2 Domain Affinity Purification and Mass Spectrometry
- Analysis of the Global Changes in SH2 Binding Properties Using Mass Spectrometry Supported by Quantitative Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technique
- Using Reciprocal Protein-Peptide Array Screening to Unravel Protein Interaction Networks
- Rosette Assay: Highly Customizable Dot-Blot for SH2 Domain Screening
- Microwestern Arrays for Systems-Level Analysis of SH2 Domain-Containing Proteins
- SH2 Binding Site Protection Assay: A Method for Identification of SH2 Domain Interaction Partners by Exploiting SH2 Mediated Phosphosite Protection
- Real Time Single Molecule Visualization of SH2 Domain Membrane Recruitment in Growth Factor Stimulated Cells
- SH2 Domain-Based FRET Biosensor for Measuring BCR-ABL Activity in Living CML Cells
- SH2 Domain Histochemistry.