Single Molecule Enzymology : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Mashanov, Gregory I. (Editor ), Batters, Christopher. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 778
Materias:
Tabla de Contenidos:
  • Single Enzyme Studies: A Historical Perspective
  • Functional Surface-Attachment in a Sandwich Geometry of GFP-Labeled Motor Proteins
  • Studying Kinesin’s Enzymatic Cycle Using a Single-Motor Confocal Motility Assay, Employing Förster Resonance Energy Transfer
  • Fluorescence Imaging with One Nanometer Accuracy: In Vitro and In Vivo Studies of Molecular Motors
  • Snapshots of Kinesin Motors on Microtubule Tracks
  • Structural and Dynamic Characterization of Biochemical Processes by Atomic Force Microscopy
  • Using Optical Tweezers to Study Fine Details of Myosin ATPase Mechanochemical Cycle
  • Quantum Dot Labeling Strategies to Characterize Single Molecular Motors
  • Imaging Individual Myosin Molecules Within Living Cells
  • Single Molecule Measurements Using Microneedles
  • Fluorescent Nucleoside Triphosphates for Single Molecule Enzymology
  • Probing the Mechanics of the Complete DNA Transcription Cycle in Real-Time Using Optical Tweezers
  • A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases
  • Real-Time Single-Molecule Observation of Green Fluorescent Protein Synthesis by Immobilized Ribosomes
  • Single-Molecule Measurements of Topoisomerase Activity with Magnetic Tweezers
  • Assembly of Recombinant Nucleosomes on Nanofabricated DNA Curtains for Single Molecule Imaging
  • Simultaneous Observation of Chemomechanical Coupling of a Molecular Motor
  • Microsecond Resolution of Single Molecule Rotation Catalyzed by Molecular Motors.