Vertebrate Embryogenesis : Embryological, Cellular, and Genetic Methods /
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| Format: | eBook |
| Language: | English |
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New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edition: | 2nd ed. 2019. |
| Series: | Methods in Molecular Biology,
1920 |
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Table of Contents:
- Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments
- Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos
- Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos
- Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts
- Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis
- Embryological and Genetic Manipulation of Chick Development
- Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos
- Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens
- RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq)
- Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos
- Ex Utero Culture and Imaging of Mouse Embryos
- Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections
- Reptile Embryology and Regeneration
- Manipulation of Developmental Function in Turtles with Notes on Alligators
- Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes
- Visualizing the Balbiani Body in Zebrafish Oocytes
- Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis
- Generation of Xenopus Haploid, Triploid, and Hybrid Embryos
- Germ Cell Transplantation in Avian Species
- Production of Germ-Line Chimeras in Zebrafish
- A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring
- Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT)
- An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish
- Modeling Embryonic Cleavage Patterns
- Laboratory Guidelines for Animal Care.