Viral Vectors for Gene Therapy : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Merten, Otto-Wilhelm. (Editor ), Al-Rubeai, Mohamed. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 737
Materias:
Tabla de Contenidos:
  • Introduction to Viral Vectors
  • Introduction to Gene Therapy: A Clinical Aftermath
  • Host Cells and Cell Banking
  • Overview of Current Scalable Methods for Purification of Viral Vectors
  • Methods to Construct Recombinant Adenovirus Vectors
  • Manufacturing of Adenoviruses Vectors: Production and Purification of Helper Dependent Adenovirus
  • Manufacturing of Retroviruses
  • Lentiviral Vectors
  • Adeno-Associated Viruses
  • Manufacturing of Adeno-Associated Viruses, for Example: AAV2
  • Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses
  • Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells
  • Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant and Amplicon Vectors
  • Manufacture of Measles Viruses
  • In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model
  • In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (in View of Cartilage Repair)
  • Ethical Consideration
  • Clinical Trials of GMP Products in the Gene Therapy Field.