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170801s2017 xxu| s |||| 0|eng d |
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|a 9781493972043
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| 024 |
7 |
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|a 10.1007/978-1-4939-7204-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a mRNA Processing :
|b Methods and Protocols /
|c edited by Yongsheng Shi.
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| 250 |
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|a 1st ed. 2017.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XI, 258 p. 49 illus., 33 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1648
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| 505 |
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|a Detection and Identification of Uncapped RNA by Ligation-Mediated Reverse Transcription Polymerase Chain Reaction -- Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay -- Single-Molecule Analysis of Pre-mRNA Splicing with Colocalization Single-Molecule Spectroscopy (CoSMoS) -- In Vitro Modulation of Endogenous Alternative Splicing Using Splice-Switching Antisense Oligonucleotides -- Purification of mRNA Processing Complexes Using an RNA Affinity Approach -- Polyadenylation Site-Based Analysis of Transcript Expression by 3’READS+ -- Comprehensive Identification of mRNA Polyadenylation Sites by PAPERCLIP -- Microinjection and Fluorescence In Situ Hybridization Assay for Studying mRNA Export in Mammalian Cells -- Methods for the Detection of Adenosine-to-Inosine Editing Events in Cellular RNA -- Using RNA-Seq to Discover Genetic Polymorphisms that Produce Hidden Splice Variants -- Inducible Expression of Eukaryotic Circular RNAs from Plasmids -- Methods for Extraction of RNA, Proteins, or Protein Complexes from Subcellular Compartments of Eukaryotic Cells -- Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine -- Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-End Crosslinking and Immunoprecipitation (seCLIP) -- Purification of Transcript-Specific mRNP Complexes Formed In Vivo from Saccharomyces cerevisiae.- A Cell-Based High-Throuput Method for Identifying Modulators of Alternative Splicing -- Genome-Wide RNAi Screens for RNA Processing Events in Drosophila Melanogaster S2 Cells -- Assaying RNA Structure Inside Living Cells with SHAPE.
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| 650 |
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|a Human genetics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 700 |
1 |
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|a Shi, Yongsheng.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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