mRNA Processing : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edición: | 1st ed. 2017. |
| Colección: | Methods in Molecular Biology,
1648 |
| Materias: |
Tabla de Contenidos:
- Detection and Identification of Uncapped RNA by Ligation-Mediated Reverse Transcription Polymerase Chain Reaction
- Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay
- Single-Molecule Analysis of Pre-mRNA Splicing with Colocalization Single-Molecule Spectroscopy (CoSMoS)
- In Vitro Modulation of Endogenous Alternative Splicing Using Splice-Switching Antisense Oligonucleotides
- Purification of mRNA Processing Complexes Using an RNA Affinity Approach
- Polyadenylation Site-Based Analysis of Transcript Expression by 3’READS+
- Comprehensive Identification of mRNA Polyadenylation Sites by PAPERCLIP
- Microinjection and Fluorescence In Situ Hybridization Assay for Studying mRNA Export in Mammalian Cells
- Methods for the Detection of Adenosine-to-Inosine Editing Events in Cellular RNA
- Using RNA-Seq to Discover Genetic Polymorphisms that Produce Hidden Splice Variants
- Inducible Expression of Eukaryotic Circular RNAs from Plasmids
- Methods for Extraction of RNA, Proteins, or Protein Complexes from Subcellular Compartments of Eukaryotic Cells
- Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine
- Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-End Crosslinking and Immunoprecipitation (seCLIP)
- Purification of Transcript-Specific mRNP Complexes Formed In Vivo from Saccharomyces cerevisiae.- A Cell-Based High-Throuput Method for Identifying Modulators of Alternative Splicing
- Genome-Wide RNAi Screens for RNA Processing Events in Drosophila Melanogaster S2 Cells
- Assaying RNA Structure Inside Living Cells with SHAPE.