Zygotic Genome Activation : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Lee, Kiho. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2017.
Edition:1st ed. 2017.
Series:Methods in Molecular Biology, 1605
Subjects:
Table of Contents:
  • Clearance of Maternal RNAs: Not a Mummy's Embryo Anymore
  • Link of Zygotic Genome Activation and Cell Cycle Control
  • Role of MicroRNAs in Zygotic Genome Activation: Modulation of mRNA during Embryogenesis
  • Gene Expression Analysis of Mammalian Oocytes and Embryos by Quantitative Real-Time RT-PCR
  • Detection of miRNA in Mammalian Oocytes and Embryos
  • Detection of Bidirectional Promoter-Derived IncRNAs from Small-Scale Samples using Pre-Amplification-Free Directional RNA-Seq Method
  • Detection and Characterization of Small Non-Coding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation
  • Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos
  • RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos
  • Detection of RNA Polymerase II in Mice Embryos during Zygotic Genome Activation using Immunocytochemistry
  • Immunological Staining of Global Changes in DNA Methylation in the Early Mammalian Embryo
  • Single Cell Restriction Enzyme-Based Analysis of Methylation at Genomic Imprinted Regions in Preimplantation Mouse Embryos
  • Use of Chemicals to Inhibit DNA Replication, Transcription, and Protein Synthesis to Study Zygotic Genome Activation
  • Targeted Gene Knockdown in Early Embryos using siRNA
  • Generating Mouse Models using Zygote Electroporation of Nucleases (ZEN) Technology with High Efficiency and Throughput
  • CRISPR/Cas9-Mediated Gene Targeting during Embryogenesis in Swine
  • Potential Involvement of SCF-Complex in Zygotic Genome Activation during Early Bovine Embryo Development
  • Use of Histone K-M Mutants for the Analysis of Transcriptional Regulation in Mouse Zygotes.