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140519s2014 xxu| s |||| 0|eng d |
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|a 9781493907991
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| 024 |
7 |
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|a 10.1007/978-1-4939-0799-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Yeast Protocols /
|c edited by Wei Xiao.
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| 250 |
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|a 3rd ed. 2014.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2014.
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| 300 |
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|a X, 313 p. 57 illus., 27 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1163
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| 505 |
0 |
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|a Basic Investigations in Saccharomyces cerevisiae -- Isolation of Yeast Nucleic Acids -- Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry -- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method -- Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe -- See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry -- Yeast Two Hybrid Liquid Screening -- Targeted Mutagenesis of a Specific Gene in Yeast -- Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae -- Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy -- Assays for Autophagy II: Mitochondrial Autophagy -- Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction -- Yeast Survival and Growth Assays -- Spontaneous Mutagenesis Assay -- Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene -- piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe -- Replicative and Chronological Life Span Assays -- Detection of Protein Arginine Methylation in Saccharomyces cerevisiae -- Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae -- Ligand Engineering Using Yeast Surface Display -- Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay.
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| 650 |
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|a Microbiology.
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| 650 |
1 |
4 |
|a Microbiology.
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| 700 |
1 |
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|a Xiao, Wei.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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