Regulatory Non-Coding RNAs Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edition: | 1st ed. 2015. |
| Series: | Methods in Molecular Biology,
1206 |
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| Online Access: | https://doi.org/10.1007/978-1-4939-1369-5 |
Table of Contents:
- Diverse Functions and Mechanisms of Mammalian Long Non-Coding RNAs
- Sequence-Defined Oligoaminoamides for the Delivery of siRNAs
- A Biochemical Approach to Identify Direct MicroRNA Targets
- Native Gel Analysis for Mammalian MicroRNPs Assembled from Pre-MicroRNAs
- Identification and Validation of miRNA Target Sites within Non-Traditional miRNA Targets
- Fractionation of Non-Polyadenylated and Ribosomal-Free RNAs from Mammalian Cells
- Affinity-Purification of Long Noncoding RNA−Protein Complexes from Formaldehyde-Cross linked Mammalian Cells
- RNA Pull down Protocol for In Vitro Detection and Identification of RNA-Associated Proteins
- nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding Protein on a Genome-Wide Scale
- Analysis of the Subcellular Distribution of RNA by Fluorescence In Situ Hybridization
- RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells
- Locating RNAs In Situ with FISH-STIC Probes
- Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisense Oligonucleotides
- On Using Magnesium and Potassium Ions in RNA Experiments
- Studying the Affinity, Kinetic Stability and Specificity of RNA/Protein Interactions: SINE ncRNA/Pol II Complexes as a Model System.