Coronaviruses Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Maier, Helena Jane. (Editor ), Bickerton, Erica. (Editor ), Britton, Paul. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:1st ed. 2015.
Colección:Methods in Molecular Biology, 1282
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-2438-7
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245 1 0 |a Coronaviruses  |b Methods and Protocols /  |c edited by Helena Jane Maier, Erica Bickerton, Paul Britton. 
250 |a 1st ed. 2015. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2015. 
300 |a XI, 285 p. 42 illus., 27 illus. in color.  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 1282 
505 0 |a Coronaviruses: An Overview of Their Replication and Pathogenesis -- Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomics -- Serological Diagnosis of Feline Coronavirus Infection by Immunochromatographic Test -- Estimation of Evolutionary Dynamics and Selection Pressure in Coronaviruses -- The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration -- The Preparation of Chicken Kidney Cell Cultures for Virus Propagation -- Isolation and Propagation of Coronaviruses in Embryonated Eggs -- Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures -- Quantification of Infectious Bronchitis Coronaviruses by Titration in vitro and in ovo -- Purification of Coronavirus Virions for Cryo-EM and Proteomic Analysis -- Partial Purification of IBV and Subsequent Isolation of Viral RNA for Next Generation Sequencing -- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses -- Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes -- Protein Histochemistry Using Coronaviral Spike Proteins: Studying Binding Profiles and Sialic Acid Requirements for Attachment to Tissues -- Identification of Protein Receptors for Coronaviruses by Mass Spectrometry -- Single Particle Tracking Assay to Study Coronavirus Membrane Fusion -- Studying Coronavirus-Host Protein Interactions -- A Field-Proven Yeast Two-Hybrid Protocol Used to Identify Coronavirus-Host Protein-Protein Interactions -- Investigation of the Functional Roles of Host Cell Proteins Involved in Coronavirus Infection Using Highly Specific and Scalable RNA Interference (RNAi) Approach -- Transcriptome Analysis of Feline Infectious Peritonitis Virus (FIPV) Infection -- Quantification of Interferon Signaling in Avian Cells -- Studying the Dynamics of Coronavirus Replicative Structures -- Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope. 
650 0 |a Microbiology. 
650 0 |a Virology. 
650 1 4 |a Microbiology. 
650 2 4 |a Virology. 
700 1 |a Maier, Helena Jane.  |e editor. 
700 1 |a Bickerton, Erica.  |e editor. 
700 1 |a Britton, Paul.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1007/978-1-4939-2438-7