|
|
|
|
LEADER |
03278nam a22003375i 4500 |
001 |
978-1-4939-2438-7 |
005 |
20191026012358.0 |
007 |
cr nn 008mamaa |
008 |
150226s2015 xxu| s |||| 0|eng d |
020 |
|
|
|a 9781493924387
|
024 |
7 |
|
|a 10.1007/978-1-4939-2438-7
|2 doi
|
040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
245 |
1 |
0 |
|a Coronaviruses
|b Methods and Protocols /
|c edited by Helena Jane Maier, Erica Bickerton, Paul Britton.
|
250 |
|
|
|a 1st ed. 2015.
|
260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
|
300 |
|
|
|a XI, 285 p. 42 illus., 27 illus. in color.
|b online resource.
|
336 |
|
|
|a text
|b txt
|2 rdacontent
|
337 |
|
|
|a computer
|b c
|2 rdamedia
|
338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
490 |
1 |
|
|a Methods in Molecular Biology,
|v 1282
|
505 |
0 |
|
|a Coronaviruses: An Overview of Their Replication and Pathogenesis -- Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomics -- Serological Diagnosis of Feline Coronavirus Infection by Immunochromatographic Test -- Estimation of Evolutionary Dynamics and Selection Pressure in Coronaviruses -- The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration -- The Preparation of Chicken Kidney Cell Cultures for Virus Propagation -- Isolation and Propagation of Coronaviruses in Embryonated Eggs -- Characterization of Human Coronaviruses on Well-Differentiated Human Airway Epithelial Cell Cultures -- Quantification of Infectious Bronchitis Coronaviruses by Titration in vitro and in ovo -- Purification of Coronavirus Virions for Cryo-EM and Proteomic Analysis -- Partial Purification of IBV and Subsequent Isolation of Viral RNA for Next Generation Sequencing -- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses -- Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes -- Protein Histochemistry Using Coronaviral Spike Proteins: Studying Binding Profiles and Sialic Acid Requirements for Attachment to Tissues -- Identification of Protein Receptors for Coronaviruses by Mass Spectrometry -- Single Particle Tracking Assay to Study Coronavirus Membrane Fusion -- Studying Coronavirus-Host Protein Interactions -- A Field-Proven Yeast Two-Hybrid Protocol Used to Identify Coronavirus-Host Protein-Protein Interactions -- Investigation of the Functional Roles of Host Cell Proteins Involved in Coronavirus Infection Using Highly Specific and Scalable RNA Interference (RNAi) Approach -- Transcriptome Analysis of Feline Infectious Peritonitis Virus (FIPV) Infection -- Quantification of Interferon Signaling in Avian Cells -- Studying the Dynamics of Coronavirus Replicative Structures -- Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope.
|
650 |
|
0 |
|a Microbiology.
|
650 |
|
0 |
|a Virology.
|
650 |
1 |
4 |
|a Microbiology.
|
650 |
2 |
4 |
|a Virology.
|
700 |
1 |
|
|a Maier, Helena Jane.
|e editor.
|
700 |
1 |
|
|a Bickerton, Erica.
|e editor.
|
700 |
1 |
|
|a Britton, Paul.
|e editor.
|
710 |
2 |
|
|a SpringerLink (Online service)
|
773 |
0 |
|
|t Springer eBooks
|
856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-4939-2438-7
|