Hedgehog Signaling Protocols
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edition: | 2nd ed. 2015. |
| Series: | Methods in Molecular Biology,
1322 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-4939-2772-2 |
Table of Contents:
- Controlled Delivery of Sonic Hedgehog with a Heparin-Based Coacervate
- In Vivo Imaging of Hedgehog Transport in Drosophila Epithelia
- Modeling Hedgehog Signaling Through Flux-Saturated Mechanism
- Evaluating the Activity of Smoothened Toward G Proteins Using [35S] Guanosine 5'-(3-O-thio)triphosphate ([35S]GTPγS)
- Analysis of Smoothened Phosphorylation and Activation in Cultured Cells and Wing Discs of Drosophila
- Investigation of Protein-Protein Interactions and Conformational Changes in Hedgehog Signaling Pathway by FRET
- Luciferase Reporter Assays to Study Transcriptional Activity of HH Signaling in Normal and Cancer Cells
- Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR
- Quantitative Immunoblotting of Endogenous Hedgehog Pathway Components
- Measuring Gli2 Phosphorylation by Selected Reaction Monitoring Mass Spectrometry
- Rapid Screening of Gli2/3 Mutants Using the Flp-In System
- Insights into Gli Factors Ubiquitylation Methods
- Determination of Acetylation of the Gli Transcription Factors
- Efficient Detection of Indian Hedgehog During Endochondral Ossification by Whole Mount Immunofluorescence
- Methods for Detection of Ptc1-Driven LacZ Expression in Adult Mouse Skin
- Determination and Analysis of Cellular Metabolic Changes by Non-Canonical Hedgehog Signaling.