Estrogen Receptors Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Eyster, Kathleen M. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1366
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-3127-9
Tabla de Contenidos:
  • The Estrogen Receptors: An Overview from Different Perspectives
  • Competitive Binding Assay for the G-Protein Coupled Receptor 30 (GPR30) or G-Protein Coupled Estrogen Receptor (GPER)
  • The Use of Real Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression
  • Bioinformatics Analysis of Estrogen-Responsive Genes
  • Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen
  • Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors
  • Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells
  • Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer
  • RNA-Seq Experiment and Data Analysis
  • DNA Microarray Analysis of Estrogen-Responsive Genes
  • Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two Dimensional Liquid Chromatography and Tandem Mass Spectrometry
  • Assessment of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate Endometrium, Placenta, and Fetal Adrenal in Response to Estrogen
  • Co-Localization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal Microscopy
  • Live Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence Microscopy
  • In Situ Hybridization of Estrogen Receptors α and β and GPER in the Human Testis
  • Purification of Histone Lysine Methyltransferase SMYD2 and Co-Crystallization with a Target Peptide from Estrogen Receptor a
  • Gold Nanoparticle-Based Förster Resonance Energy Transfer (FRET) Analysis of Estrogen Receptor: DNA Interaction
  • Analysis of Interaction of Estradiol with Estrogen Receptor by NMR Spectroscopy
  • Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction between the Estrogen Receptor Alpha and Co-Activators: The Case of Calmodulin
  • Surface Plasmon Resonance Study of Cooperative Interactions of Estrogen Receptor α and Specificity Protein 1 with Composite DNA Elements
  • Tips and Tricks for Successful Application of Statistical Methods to Biological Data
  • The Synonymous Ala87 Mutation of Estrogen Receptor Alpha Modifies Transcriptional Activation through Both ERE and AP1 Sites
  • Selective Estrogen Receptor Modulators and the Tissue-Selective Estrogen Complex: Analysis of Cell Type-Specific Effects using In Vivo Imaging of a Reporter Mouse Model
  • Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model
  • Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo Culture
  • Estrogen Receptor Agonists and Antagonists in the Yeast Estrogen Bioassay
  • Silencing Estrogen Receptor-α with siRNA in the Intact Rodent Brain
  • Silencing Estrogen Receptor-β with siRNA in Cultured Cells
  • Experimental Procedures for Demonstration of MicroRNA Mediated Enhancement of Functional Neuroprotective Effects of Estrogen Receptor Agonists
  • Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer Cells
  • Estradiol-Induced Transcriptional Regulation of Long Non-Coding RNA, HOTAIR.-Detection and Functional Analysis of Estrogen Receptor α Phosphorylated at Serine 216 in Mouse Neutrophils
  • Estrogen Receptor-α Knockout Mice
  • Detection of Endogenous Selective Estrogen Receptor Modulators such as 27-Hydroxycholesterol
  • Phytoestrogens Activate the Estrogen Receptor in HepG2 Cells
  • Detection of the Phosphorylation of the Estrogen Receptor a as an Outcome of GPR30 Activation
  • GPER Mediates Non-Genomic Effects of Estrogen
  • GPER/GPR30 Knockout Mice: Effects of GPER on Metabolism
  • Analysis of G-Protein Coupled Receptor 30 (GPR30) on Endothelial Inflammation
  • Atherosclerosis and Vascular Biologic Responses to Estrogens: Histologic, Immunohistochemical, Biochemical, and Molecular Methods
  • Assessing Direct Vascular Actions of Estrogens
  • Molecular Design, Synthesis, and Evaluation of SNIPER(ER) that Induces Proteasomal Degradation of ERα
  • Regulation of Activation Induced Deaminase (AID) by Estrogen.