Lipid Signaling Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Waugh, Mark. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2016.
Edition:2nd ed. 2016.
Series:Methods in Molecular Biology, 1376
Subjects:
Online Access:https://doi.org/10.1007/978-1-4939-3170-5
Table of Contents:
  • Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format
  • Assaying Ceramide Synthase Activity In Vitro and in Living Cells using Liquid Chromatography-Mass Spectrometry
  • Fluorescent Assays for Ceramide Synthase Activity
  • Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-kinase type II Alpha
  • Measurement of Long Chain Fatty Acyl-CoA synthetase Activity
  • Qualitative and Quantitative in vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specificity
  • Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity
  • Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Microdomains
  • Fluorescence Recovery after Photobleaching Analysis of the Diffusional Mobility of Plasma Membrane Proteins: HER3 Mobility in Breast Cancer Cell Membranes
  • Isolation and Analysis of Detergent-Resistant Membrane Fractions
  • Detection of Isolated Mitochondria-Associated ER Membranes using the Sigma-1 Receptor
  • Using Surface Plasmon Resonance to Quantitatively Assess Lipid-Protein Interactions
  • Analyzing Protein-phosphoinositide Interactions with Liposome Flotation Assays
  • High-Throughput Flurometric Assay for Membrane-Protein Interaction
  • Guidelines for the use of Protein Domains in Acidic Phospholipid Imaging
  • Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [3H]serine
  • Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells
  • Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions
  • Single-molecule Imaging of Signal Transduction via GPI-anchored Receptors
  • Measuring Phosphatidylinositol Generation on Biological Membranes
  • Assay for CDP-diacylglycerol Generation by CDS in Membrane Fractions.