Molecular Genetics of Asthma

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Isidoro García, María. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1434
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-3652-6
Tabla de Contenidos:
  • Applications of Molecular Genetics to the Study of Asthma
  • Assessment and Validation of New Genetic Variants: A systematic In Silico Approach
  • Introduction to the Gene Expression Analysis
  • Real Time PCR for Gene Expression Quantification in Asthma
  • Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma
  • Review of Methods to Study Gene Expression Regulation Applied to Asthma
  • Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma
  • SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma
  • Chromatin Immunoprecipitation: Application to the Study of Asthma
  • Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols
  • Protocols for Exosome Isolation and RNA Profiling
  • Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell
  • Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line
  • Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line
  • Review of Mouse Models Applied to the Study of Asthma
  • General and Specific Mouse Models for Asthma Research
  • Mouse Models applied to the Research of Pharmacological Treatments in Asthma
  • Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma.