Human Respiratory Syncytial Virus Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Tripp, Ralph A. (Editor ), Jorquera, Patricia A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1442
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-3687-8
Tabla de Contenidos:
  • Human Respiratory Syncytial Virus: An Introduction
  • RSV Growth and Quantification by Microtitration and qRT-PCR Assays
  • Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay
  • Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
  • Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cells
  • Development of Human Monoclonal Antibodies against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique
  • Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection
  • Host Factors Modulating RSV Infection; Use of Small Interfering RNAs to Probe Functional Importance
  • In Vitro Modelling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs)
  • Reverse Genetics of Respiratory Syncytial Virus
  • Use of Minigenome Systems to Study RSV Transcription
  • Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics
  • A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellular Factors as Drug Targets
  • MicroRNA Profiling from RSV Infected Biofluids, Whole Blood, and Tissue Samples
  • Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus
  • In Vivo Assessment of Airway Function in the Mouse Model
  • Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus. .