Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Federico, Maurizio. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1448
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-3753-0
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245 1 0 |a Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools  |c edited by Maurizio Federico. 
250 |a 1st ed. 2016. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2016. 
300 |a XI, 276 p. 47 illus., 33 illus. in color.  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 1448 
505 0 |a Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown -- Development of Inducible Molecular Switches Based on All-In-One Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase -- Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions -- Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G -- Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors -- Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors -- Production and Concentration of Lentivirus for Transduction of Primary Human T Cells -- Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer -- The LAM-PCR Method to Sequence LV Integration Sites -- Conditional Rnai using the Lentiviral GLTR System -- Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies -- Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line -- Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease -- Development of Lentiviral Vectors for Targeted Integration and Protein Delivery -- Biogenesis and Functions of Exosomes and Extracellular Vesicles -- Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes -- Cardiac Myocyte Exosome Isolation -- Incorporation of Heterologous Proteins in Engineered Exosomes -- Exosome-Mediated Targeted Delivery of Mirnas. 
650 0 |a Human genetics. 
650 1 4 |a Human Genetics. 
700 1 |a Federico, Maurizio.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1007/978-1-4939-3753-0