Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Federico, Maurizio. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1448
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-3753-0
Tabla de Contenidos:
  • Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown
  • Development of Inducible Molecular Switches Based on All-In-One Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase
  • Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions
  • Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G
  • Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors
  • Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors
  • Production and Concentration of Lentivirus for Transduction of Primary Human T Cells
  • Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer
  • The LAM-PCR Method to Sequence LV Integration Sites
  • Conditional Rnai using the Lentiviral GLTR System
  • Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies
  • Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line
  • Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease
  • Development of Lentiviral Vectors for Targeted Integration and Protein Delivery
  • Biogenesis and Functions of Exosomes and Extracellular Vesicles
  • Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes
  • Cardiac Myocyte Exosome Isolation
  • Incorporation of Heterologous Proteins in Engineered Exosomes
  • Exosome-Mediated Targeted Delivery of Mirnas.