High-Throughput RNAi Screening Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Azorsa, David O. (Editor ), Arora, Shilpi. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1470
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-6337-9
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245 1 0 |a High-Throughput RNAi Screening  |b Methods and Protocols /  |c edited by David O. Azorsa, Shilpi Arora. 
250 |a 1st ed. 2016. 
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300 |a XI, 262 p. 52 illus., 43 illus. in color.  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 1470 
505 0 |a Establishing an Infrastructure for High-Throughput Short-Interfering RNA Screening -- Optimization of Transfection Conditions for siRNA Screening -- High Throughput siRNA Screening Using Reverse Transfection -- Genome-Wide siRNA Screening Using Forward Transfection: Identification of Modulators of Membrane Trafficking in Mammalian Cells -- Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform -- A Protocol for a High-Throughput Multiplex Cell Viability Assay -- RNAi Screening of Leukemia Cells Using Electroporation -- Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening -- In Vitro Pooled shRNA Screening to Identify Determinants of Radiosensitivity -- Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens -- In Vitro High Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development -- High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans -- Design and Methods of Large-Scale RNA Interference Screens in Drosophila -- Genome-Wide RNAi Screening in C. elegans for Aging and Innate Immunity -- RNAi-Assisted Genome Evolution (RAGE) in Saccharomyces cerevisiae -- RNAi Screening in Spodoptera frugiperda -- Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos -- Live Cell Microscopy-Based RNAi Screening in the Moss Physcomitrella patens -- Data Analysis For High Throughput RNAi Screening. . 
650 0 |a Human genetics. 
650 1 4 |a Human Genetics. 
700 1 |a Azorsa, David O.  |e editor. 
700 1 |a Arora, Shilpi.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1007/978-1-4939-6337-9