High-Throughput RNAi Screening Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Azorsa, David O. (Editor ), Arora, Shilpi. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1470
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-6337-9
Tabla de Contenidos:
  • Establishing an Infrastructure for High-Throughput Short-Interfering RNA Screening
  • Optimization of Transfection Conditions for siRNA Screening
  • High Throughput siRNA Screening Using Reverse Transfection
  • Genome-Wide siRNA Screening Using Forward Transfection: Identification of Modulators of Membrane Trafficking in Mammalian Cells
  • Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform
  • A Protocol for a High-Throughput Multiplex Cell Viability Assay
  • RNAi Screening of Leukemia Cells Using Electroporation
  • Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening
  • In Vitro Pooled shRNA Screening to Identify Determinants of Radiosensitivity
  • Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens
  • In Vitro High Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development
  • High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans
  • Design and Methods of Large-Scale RNA Interference Screens in Drosophila
  • Genome-Wide RNAi Screening in C. elegans for Aging and Innate Immunity
  • RNAi-Assisted Genome Evolution (RAGE) in Saccharomyces cerevisiae
  • RNAi Screening in Spodoptera frugiperda
  • Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos
  • Live Cell Microscopy-Based RNAi Screening in the Moss Physcomitrella patens
  • Data Analysis For High Throughput RNAi Screening. .