CCN Proteins Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Takigawa, Masaharu. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1489
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-6430-7
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245 1 0 |a CCN Proteins  |b Methods and Protocols /  |c edited by Masaharu Takigawa. 
250 |a 1st ed. 2017. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2017. 
300 |a XV, 576 p. 104 illus., 67 illus. in color.  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 1489 
505 0 |a The CCN Proteins: An Overview -- Gene Expression Analysis of CCN Proteins: Whole Mount In Situ Hybridization Of CCN2 in Developing Calcified Tissues -- Expression of CCN Genes and Proteins in Human Skin: Methods And Protocols -- Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cells -- Western Blotting Analysis of CCN Proteins -- Immunohistochemical Analysis of CCN Proteins -- Analysis of CCN Expression by Immunofluorescence on Skin Cells, Skin, and Reconstructed Epidermis -- Production of Recombinant CCN2 Protein in Escherichia. Coli -- Production Of Recombinant CCN Proteins By Brevibacillus Choshinensis -- Production Of Recombinant CCN2 Protein By Mammalian Cells -- In Vitro Transfection with and Expression of CCN Family of Genes -- Preparation of Module Specific Antibodies against CCN2 -- ELISA of CCN Proteins in Body Fluids Including Serum And Plasma -- Analysis of Signaling Pathways Activated by CCN Proteins -- Protocols for Screening for Binding Partners of CCN Proteins: Yeast Two- Hybrid System -- Protocols For Screening Peptide Motifs Binding To CCN Proteins -- Evaluation Of Molecular Interaction Between CCN Proteins and its Binding Partners By Surface Plasmon Resonance (SPR) -- Promoter Analysis of CCN Genes -- Analysis Of Posttranscriptional Regulation Of CCN Genes -- Protein Imaging of CCN2 and CCN3 in Living Cells -- Cell Biological Assays for Measuring Chondrogenic Activities of CCN Proteins -- Protocol for Measuring Angiogenic Activities of CCN Proteins -- Cell Biological Assays for Measuring Odontogenic Activities of CCN Proteins -- Separation and Enrichment of Haematopoietic Stem Cells for CCN Studies -- In Vivo Evaluation of Cartilage Regenerative Effects of CCN Proteins -- Gene Expression Analysis of CCN Proteins in Bone Under Mechanical Stress -- The Bone Regeneration Model and Primary Osteoblastic Cell Culture Used in the Analysis of CCN3 Transgenic and Knockout Mice -- Design and Analysis of CCN Gene Activity Using CCN Knockout Mice Containing Lacz Reporters -- Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches -- Construction and Analysis of an Allelic Series of CCN1 Knockin Mice -- Production and Analysis of Conditional KO Mice of CCN2 in Kidney -- Generation And Analysis Of Cartilage-Specific CCN2 Overexpression In Transgenic Mice -- Analysis of Transcytosis of CCN2 by Chondrocytes -- CCN2 in Skin Fibrosis -- Studying the CCN Proteins in Fibrosis -- Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases - Kidney Fibrosis -- Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases - Liver Fibrosis -- Cellular or Exosomal Micrornas Associated with CCN Gene Expression in Liver Fibrosis -- TGF-Β1 snd CCN2-Stimulated Sirius Red Assay for Collagen Accumulation in Cultured Cells -- CCN Detection of Cancer Tissues by Immunohistochemistry Staining -- Gene Expression Analysis of CCN Proteins: Detection of CCN1 and CCN5 MRNA in Human Cancer Samples using a Modified In Situ Hybridization Technique -- Analysis of Pathological Activities of CCN Proteins in Bone Metastasis -- Analysis of Pathological Activities of CCN2/CTGF in Muscle Dystrophy -- Method for Analysis of Matrix Degradation by CCN2 through the MMP/TIMP System -- An Analysis of Pathological Activities of CCN Proteins in Joint Disorders−Mechanical Stretch-Mediated CCN2 Expression in Cultured Meniscus Cells− -- Analysis of CCN Protein Expression and Activities in Vasoproliferative Retinopathies. . 
650 0 |a Proteins . 
650 1 4 |a Protein Science. 
700 1 |a Takigawa, Masaharu.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1007/978-1-4939-6430-7