CCN Proteins Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Takigawa, Masaharu. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1489
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-6430-7
Tabla de Contenidos:
  • The CCN Proteins: An Overview
  • Gene Expression Analysis of CCN Proteins: Whole Mount In Situ Hybridization Of CCN2 in Developing Calcified Tissues
  • Expression of CCN Genes and Proteins in Human Skin: Methods And Protocols
  • Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cells
  • Western Blotting Analysis of CCN Proteins
  • Immunohistochemical Analysis of CCN Proteins
  • Analysis of CCN Expression by Immunofluorescence on Skin Cells, Skin, and Reconstructed Epidermis
  • Production of Recombinant CCN2 Protein in Escherichia. Coli
  • Production Of Recombinant CCN Proteins By Brevibacillus Choshinensis
  • Production Of Recombinant CCN2 Protein By Mammalian Cells
  • In Vitro Transfection with and Expression of CCN Family of Genes
  • Preparation of Module Specific Antibodies against CCN2
  • ELISA of CCN Proteins in Body Fluids Including Serum And Plasma
  • Analysis of Signaling Pathways Activated by CCN Proteins
  • Protocols for Screening for Binding Partners of CCN Proteins: Yeast Two- Hybrid System
  • Protocols For Screening Peptide Motifs Binding To CCN Proteins
  • Evaluation Of Molecular Interaction Between CCN Proteins and its Binding Partners By Surface Plasmon Resonance (SPR)
  • Promoter Analysis of CCN Genes
  • Analysis Of Posttranscriptional Regulation Of CCN Genes
  • Protein Imaging of CCN2 and CCN3 in Living Cells
  • Cell Biological Assays for Measuring Chondrogenic Activities of CCN Proteins
  • Protocol for Measuring Angiogenic Activities of CCN Proteins
  • Cell Biological Assays for Measuring Odontogenic Activities of CCN Proteins
  • Separation and Enrichment of Haematopoietic Stem Cells for CCN Studies
  • In Vivo Evaluation of Cartilage Regenerative Effects of CCN Proteins
  • Gene Expression Analysis of CCN Proteins in Bone Under Mechanical Stress
  • The Bone Regeneration Model and Primary Osteoblastic Cell Culture Used in the Analysis of CCN3 Transgenic and Knockout Mice
  • Design and Analysis of CCN Gene Activity Using CCN Knockout Mice Containing Lacz Reporters
  • Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches
  • Construction and Analysis of an Allelic Series of CCN1 Knockin Mice
  • Production and Analysis of Conditional KO Mice of CCN2 in Kidney
  • Generation And Analysis Of Cartilage-Specific CCN2 Overexpression In Transgenic Mice
  • Analysis of Transcytosis of CCN2 by Chondrocytes
  • CCN2 in Skin Fibrosis
  • Studying the CCN Proteins in Fibrosis
  • Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases - Kidney Fibrosis
  • Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases - Liver Fibrosis
  • Cellular or Exosomal Micrornas Associated with CCN Gene Expression in Liver Fibrosis
  • TGF-Β1 snd CCN2-Stimulated Sirius Red Assay for Collagen Accumulation in Cultured Cells
  • CCN Detection of Cancer Tissues by Immunohistochemistry Staining
  • Gene Expression Analysis of CCN Proteins: Detection of CCN1 and CCN5 MRNA in Human Cancer Samples using a Modified In Situ Hybridization Technique
  • Analysis of Pathological Activities of CCN Proteins in Bone Metastasis
  • Analysis of Pathological Activities of CCN2/CTGF in Muscle Dystrophy
  • Method for Analysis of Matrix Degradation by CCN2 through the MMP/TIMP System
  • An Analysis of Pathological Activities of CCN Proteins in Joint Disorders−Mechanical Stretch-Mediated CCN2 Expression in Cultured Meniscus Cells−
  • Analysis of CCN Protein Expression and Activities in Vasoproliferative Retinopathies. .