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03195nam a22003015i 4500 |
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978-1-4939-6824-4 |
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20191023161507.0 |
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cr nn 008mamaa |
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170308s2017 xxu| s |||| 0|eng d |
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|a 9781493968244
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|a 10.1007/978-1-4939-6824-4
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Mitochondria
|b Practical Protocols /
|c edited by Dejana Mokranjac, Fabiana Perocchi.
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| 250 |
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|a 2nd ed. 2017.
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| 260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XI, 409 p. 77 illus., 46 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1567
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| 505 |
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|a A Guide to Computational Methods for Predicting Mitochondrial Localization -- Isolation of Functional Mitochondria from Cultured Cells and Mouse Tissues -- Isolation of Mitochondria From Saccharomyces Cerevisiae -- Isolation of Contact Sites Between Inner and Outer Mitochondrial Membranes -- Isolation Of Mitochondria-Associated Membranes (MAM) from Mouse Brain Tissue -- Label-Free Quantitative Analysis of Mitochondrial Proteomes using the Multienzyme Digestion-Filter Aided Sample Preparation (MED-FASP) and ‘Total Protein Approach’ -- Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry -- Detection of Cysteine Redox States in Mitochondrial Proteins in Intact Mammalian Cells -- Chemical Crosslinking in Intact Mitochondria -- Reconstitution of Mitochondrial Membrane Proteins into Nanodiscs by Cell-Free Expression -- Detection of Dual Targeting and Dual Function of Mitochondrial Proteins in Yeast -- Localizing Mrnas Encoding Mitochondrial Proteins in Yeast by Fluorescence Microscopy and Subcellular Fractionation -- Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Various Mouse Tissues using Seahorse XF96 Analyzer -- Application of FRET-Based Biosensor “Ateam” for Visualization of ATP Levels in the Mitochondrial Matrix of Living Mammalian Cells -- A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake -- New Imaging Tools to Analyze Mitochondrial Morphology in Caenorhabditis Elegans -- Single Molecule Tracking and Localization Of Mitochondrial Protein Complexes in Live Cells -- Analysis of Yeast Mitochondria by Electron Microscopy -- Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-Tomography -- Assays for Mitophagy in Yeast -- Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis Elegans -- Assessing Mitochondrial Unfolded Protein Response in Mammalian Cells -- Analysis of Mitochondrial RNA-Processing Defects in Patient-Derived Tissues by Qrt-PCR and Rnaseq -- Identification of Disease-Causing Mutations by Functional Complementation of Patient-Derived Fibroblast Cell Lines. .
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Mokranjac, Dejana.
|e editor.
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| 700 |
1 |
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|a Perocchi, Fabiana.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-1-4939-6824-4
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