Membrane Protein Structure and Function Characterization Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Lacapere, Jean-Jacques. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1635
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-4939-7151-0
Tabla de Contenidos:
  • Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2
  • Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex
  • Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris protoplasts
  • Cell-free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-synthase subunits
  • Wheat Germ Cell Free Overexpression for the Production of Membrane Proteins
  • Methyl-specific Isotope Labelling Strategies for NMR Studies of Membrane Proteins
  • Labeling of Membrane Complexes for Electron Microscopy
  • Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins Case Study of The Mouse 5-HT Receptor
  • Characterization of New Detergent and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization
  • Secondary Structure Determination by Means of ATR-FTIR Spectroscopy
  • Native Mass Spectroscopy for the Characterization of Structure and Interactions of Membrane Proteins
  • Mass Spectroscopy of Mitochondrial Membrane Proteins Complexes
  • Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+NQR from V cholerae in the Presence of Lipids
  • Reconstitution of Membrane Protein in Liposomes
  • Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes
  • The CRACAM Robot: Two Dimensional Crystallization of Membrane Protein
  • Reconstitution of Membrane Proteins into Nanodiscs for Single Particle Electron Microscopy
  • Solid-state NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO
  • Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR
  • Simulation of Ligand Binding To Membrane Proteins
  • Molecular modelling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO Molecular Dynamic of Membrane Protein in Different Environments. .