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02335nam a22002895i 4500 |
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978-1-4939-7223-4 |
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|a 9781493972234
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|a 10.1007/978-1-4939-7223-4
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Mammalian Synthetic Promoters
|c edited by David Gould.
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|a 1st ed. 2017.
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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|a XI, 277 p. 43 illus., 25 illus. in color.
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
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|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1651
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|a Initial Considerations before Designing a Promoter Construct -- Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay -- Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter -- Secreted Reporters for Monitoring Multiple Promoter Function -- Bioluminescence Monitoring Of Promoter Activity In Vitro and In Vivo -- Monitoring Promoter Activity by Flow Cytometry -- Functional Screening of Core Promoter Activity -- Bioinformatically Informed Design of Synthetic Mammalian Promoters -- Synthetic Tumor Specific Promoters for Transcriptional Regulation of Viral Replication -- Constructing Strong Cell-Type-Specific Promoters through Informed Design -- PCR Assembly of Synthetic Promoters -- The Tetracycline Responsive System -- Light-responsive Promoters -- A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation -- Promoter Activation with Electromagnetism -- Application of Synthetic Tumor Specific Promoters Responsive to the Tumor Microenvironment -- A Modular Approach to Building Complex Synthetic Circuits -- Computational Sequence Design with R2oDNA Designer -- Design of Synthetic Promoters for Gene Circuits in Mammalian Cells. .
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|a Bioinformatics.
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|a Bioinformatics.
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|a Gould, David.
|e editor.
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|a SpringerLink (Online service)
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|t Springer eBooks
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|u https://doi.org/10.1007/978-1-4939-7223-4
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