Mammalian Synthetic Promoters
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edition: | 1st ed. 2017. |
| Series: | Methods in Molecular Biology,
1651 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-4939-7223-4 |
Table of Contents:
- Initial Considerations before Designing a Promoter Construct
- Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay
- Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter
- Secreted Reporters for Monitoring Multiple Promoter Function
- Bioluminescence Monitoring Of Promoter Activity In Vitro and In Vivo
- Monitoring Promoter Activity by Flow Cytometry
- Functional Screening of Core Promoter Activity
- Bioinformatically Informed Design of Synthetic Mammalian Promoters
- Synthetic Tumor Specific Promoters for Transcriptional Regulation of Viral Replication
- Constructing Strong Cell-Type-Specific Promoters through Informed Design
- PCR Assembly of Synthetic Promoters
- The Tetracycline Responsive System
- Light-responsive Promoters
- A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation
- Promoter Activation with Electromagnetism
- Application of Synthetic Tumor Specific Promoters Responsive to the Tumor Microenvironment
- A Modular Approach to Building Complex Synthetic Circuits
- Computational Sequence Design with R2oDNA Designer
- Design of Synthetic Promoters for Gene Circuits in Mammalian Cells. .