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03435nam a22003135i 4500 |
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978-1-4939-9450-2 |
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20191024011649.0 |
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cr nn 008mamaa |
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190530s2019 xxu| s |||| 0|eng d |
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|a 9781493994502
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|a 10.1007/978-1-4939-9450-2
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Antigen Processing
|b Methods and Protocols /
|c edited by Peter van Endert.
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| 250 |
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|a 2nd ed. 2019.
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| 260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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| 300 |
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|a XV, 458 p. 79 illus., 45 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1988
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|a Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes -- Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry -- Trimming of MHC Class I Ligands by ERAP Aminopeptidases -- Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases -- Testing the Impact of Protease Inhibitors in Antigen Presentation Assays -- In Vitro Studies of MHC Class I Peptide Loading and Exchange -- Measuring Synthesis and Degradation of MHC Class I Molecules -- Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules -- A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics -- Purification and Identification of Naturally Presented MHC Class I and II Ligands -- Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs -- Quantitating MHC Class I Ligand Production and Presentation using TCR-Like Antibodies -- Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors -- A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway -- Evaluating CD8+ T Cell Responses In Vitro -- Recombinant Poxviruses: Versatile Tools for Immunological Assays -- Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy -- Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases -- Monitoring MHC-II Endocytosis and Recycling using Cell-Surface Protein Biotinylation-Based Assays -- Monitoring Protein Endocytosis and Recycling using FACS-Based Assays -- In Vitro Digestion with Proteases Producing MHC Class II Ligands -- Assembly, Intracellular Transport and Release of MHC Class II Peptide Receptors -- Pulse-Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading -- Studying MHC Class II Peptide Loading and Editing In Vitro -- Monitoring Antigen Processing for MHC Presentation via Macroautophagy -- Identification of Human Antigen-Specific CD4+ T-Cells with Peptide-MHC Multimer Technologies -- Production of Antigen Specific Human CD4+ T Cell Lines and Clones -- Monitoring Dendritic Cell Activation and Maturation -- Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes -- Analyzing Antigen Recognition by Natural Killer T Cells.
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| 650 |
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|a Molecular biology.
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| 650 |
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|a Antibodies.
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| 650 |
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4 |
|a Molecular Medicine.
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| 650 |
2 |
4 |
|a Antibodies.
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| 700 |
1 |
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|a van Endert, Peter.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-1-4939-9450-2
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