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02910nam a22003375i 4500 |
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978-1-4939-9635-3 |
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20191025171943.0 |
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cr nn 008mamaa |
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190621s2019 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493996353
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| 024 |
7 |
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|a 10.1007/978-1-4939-9635-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Antiviral Resistance in Plants
|b Methods and Protocols /
|c edited by Kappei Kobayashi, Masamichi Nishiguchi.
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| 250 |
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|a 1st ed. 2019.
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| 260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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| 300 |
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|a XI, 292 p. 37 illus., 27 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 2028
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| 505 |
0 |
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|a Identification and Functional Analysis of NB-LRR-Type Virus Resistance Genes—Overview and Functional Analysis of Candidate Genes -- Insertion of Epitope Tag into NB-LRR Class Plant Virus Resistance Protein -- Reverse genetic analysis of antiviral resistance signaling and the resistance mechanism in Arabidopsis thaliana -- Analysis of Antiviral Resistance Signaling Pathways by Virus-Induced Gene Silencing in Nicotiana benthamiana -- Random Mutagenesis of Virus Gene for the Experimental Evaluation of the Durability of NB-LRR Class Plant Virus Resistance Gene -- A Cell-free Replication System for Positive-strand RNA Viruses for Identification and Characterization of Plant Resistance Gene Products -- Plant Protein-Mediated Inhibition of Virus Cell-to-Cell Movement —Far-western Screening and Biological Analysis of a Plant Protein Interacting with a Viral Movement Protein— -- Transfection of Protoplasts Prepared from Arabidopsis thaliana Leaves for Plant Virus Research -- CRISPR/Cas9-mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Potato Plants -- A Simplified Method to Engineer CRISPR/Cas9-Mediated Geminivirus Resistance in Plants -- Computational Workflow for Small RNA Profiling in Virus-Infected Plants -- A Sensitized Genetic Screen to Identify Novel Components and Regulators of the Host Antiviral RNA Interference Pathway -- Design, Synthesis and Functional Analysis of Highly Specific Artificial Small RNAs With Antiviral Activity in Plants -- Resistance Breeding through RNA Silencing of Host Factor Involved in Virus Replication -- Large-Scale Inoculation and Evaluation Methods for Attenuated Plant Viruses -- RNA Silencing-Mediated Apple Latent Spherical Virus Vaccine in Plants.
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| 650 |
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|a Plant science.
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| 650 |
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|a Botany.
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| 650 |
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|a Virology.
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| 650 |
1 |
4 |
|a Plant Sciences.
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| 650 |
2 |
4 |
|a Virology.
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| 700 |
1 |
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|a Kobayashi, Kappei.
|e editor.
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| 700 |
1 |
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|a Nishiguchi, Masamichi.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-4939-9635-3
|