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03018nam a22003495i 4500 |
| 001 |
978-1-4939-9748-0 |
| 005 |
20191025041415.0 |
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cr nn 008mamaa |
| 008 |
190826s2020 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493997480
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| 024 |
7 |
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|a 10.1007/978-1-4939-9748-0
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Cryptosporidium
|b Methods and Protocols /
|c edited by Jan R. Mead, Michael J. Arrowood.
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| 250 |
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|a 1st ed. 2020.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2020.
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| 300 |
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|a XI, 407 p. 93 illus., 71 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 2052
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| 505 |
0 |
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|a Cryptosporidium Diagnostic Assays: Microscopy -- Cryptosporidium Diagnostic Assays: Molecular Detection -- Detection of Cryptosporidium Recovered From Large-Volume Water Samples Using Dead-End Ultrafiltration -- Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation -- Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay -- Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii -- Cryptosporidium Genotyping for Epidemiology Tracking -- Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection -- Preparation of Cryptosporidium DNA for Whole Genome Sequencing -- Accessing Cryptosporidium Omic and Isolate Data Via CryptoDB.org -- A Novel Method to Silence Genes in Cryptosporidium -- Use of miRNAs to Study Host Cell-Parasite Interactions -- Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9 -- Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology -- Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics -- Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water -- High-Content Screening for Cryptosporidium Drug Discovery -- High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR -- In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds -- In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers -- Two and Three Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium.
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| 650 |
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0 |
|a Parasitology.
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| 650 |
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0 |
|a Microbiology.
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| 650 |
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0 |
|a Infectious diseases.
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| 650 |
1 |
4 |
|a Parasitology.
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| 650 |
2 |
4 |
|a Microbiology.
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| 650 |
2 |
4 |
|a Infectious Diseases.
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| 700 |
1 |
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|a Mead, Jan R.
|e editor.
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| 700 |
1 |
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|a Arrowood, Michael J.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-4939-9748-0
|