Transforming Growth Factor-Beta Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Howe, Philip H. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2000.
Edición:1st ed. 2000.
Colección:Methods in Molecular Biology, 142
Materias:
Acceso en línea:https://doi.org/10.1385/1592590535
Tabla de Contenidos:
  • In Vitro Assays for Measuring TGF? Growth Stimulation and Inhibition
  • Measurements of Active TGF? Generated by Culture Cells
  • Methods for Iodination of Active TGF?, TGF? Receptor Crosslinking and Immunoprecipitation of TGF?-Receptor Complexes
  • Detection of TGF? in Body Fluids and Tissues
  • Analysis of TGF?-Mediated Synthesis of Extracellular Matrix Components
  • Characterization of SMAD Phosphorylation and SMAD/Receptor Interaction
  • Promoter Analysis of TGF?-Responsive Genes by Transient Transfection and Deletional/Mutational Analysis
  • Regulation of AP-1 Activity by TGF?
  • CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors
  • Activation of the Mitogen-Activated Protein Kinase Pathway by TGF?
  • 11 Detection of TGF? Type II Receptor Hotspot Mutations: The BAT-RII Assay
  • Mutation Detection in the TGF? Receptors and SMAD Genes: RT-PCR and Sequencing
  • Analysis of TGF?-Inducible Apoptosis.