Transforming Growth Factor-Beta Protocols
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2000.
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| Edition: | 1st ed. 2000. |
| Series: | Methods in Molecular Biology,
142 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592590535 |
Table of Contents:
- In Vitro Assays for Measuring TGF? Growth Stimulation and Inhibition
- Measurements of Active TGF? Generated by Culture Cells
- Methods for Iodination of Active TGF?, TGF? Receptor Crosslinking and Immunoprecipitation of TGF?-Receptor Complexes
- Detection of TGF? in Body Fluids and Tissues
- Analysis of TGF?-Mediated Synthesis of Extracellular Matrix Components
- Characterization of SMAD Phosphorylation and SMAD/Receptor Interaction
- Promoter Analysis of TGF?-Responsive Genes by Transient Transfection and Deletional/Mutational Analysis
- Regulation of AP-1 Activity by TGF?
- CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors
- Activation of the Mitogen-Activated Protein Kinase Pathway by TGF?
- 11 Detection of TGF? Type II Receptor Hotspot Mutations: The BAT-RII Assay
- Mutation Detection in the TGF? Receptors and SMAD Genes: RT-PCR and Sequencing
- Analysis of TGF?-Inducible Apoptosis.