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03223nam a22002895i 4500 |
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978-1-59259-054-4 |
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20191026221454.0 |
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cr nn 008mamaa |
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100301s2000 xxu| s |||| 0|eng d |
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|a 9781592590544
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024 |
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|a 10.1385/1592590543
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Stress Response
|b Methods and Protocols /
|c edited by Stephen M. Keyse.
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|a 1st ed. 2000.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2000.
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|a XVI, 488 p.
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 99
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|a Detection and Assay of Stress-Induced Damage -- Identifying and Counting Protein Modifications Triggered by Nitrosative Stress -- Determination of Carbonyl Groups in Oxidized Proteins -- Quantitation of 4-Hydroxynonenal Protein Adducts -- Detection of Oxidative Stress in Lymphocytes Using Dichlorodihydrofluorescein Diacetate -- The Measurement of Protein Degradation in Response to Oxidative Stress -- The Activation of Signal Transduction by Cellular Stress -- Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress -- Detection and Activation of Stress-Responsive Tyrosine Kinases -- Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells -- Expression and Assay of Recombinant ATM -- Detection and Purification of a Multiprotein Kinase Complex from Mammalian Cells -- Methods to Assay Stress-Activated Protein Kinases -- Monitoring the Activation of Stress-Activated Protein Kinases Using GAL4 Fusion Transactivators -- Use of Kinase Inhibitors to Dissect Signaling Pathways -- The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation -- Peptide Assay of Protein Kinases and Use of Variant Peptides to Determine Recognition Motifs -- The Analysis of Stress-Induced Gene Expression -- Assaying NF-KB and AP-1 DNA-Binding and Transcriptional Activity -- Analysis of the Mammalian Heat-Shock Response -- Approaches to Define the Involvement of Reactive Oxygen Species and Iron in Ultraviolet-A Inducible Gene Expression -- The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression -- Sage: The Serial Analysis of Gene Expression -- Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol -- UVB-Regulated Gene Expression in Human Keratinocytes -- Analysis of Stress Protein Function -- Heme Oxygenase Activity Current Methods and Applications -- Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches -- Analysis of Chaperone Properties of Small Hsp’ -- Analysis of Small Hsp Phosphorylation -- Analysis of Multisite Phosphorylation of the p53 Tumor-Suppressor Protein byTryptic Phosphopeptide Mappin -- Development of Physiological Models to Study Stress Protein Responses.
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|a Cell biology.
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650 |
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|a Cell Biology.
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|a Keyse, Stephen M.
|e editor.
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710 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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|u https://doi.org/10.1385/1592590543
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