Stress Response Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Keyse, Stephen M. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2000.
Edición:1st ed. 2000.
Colección:Methods in Molecular Biology, 99
Materias:
Acceso en línea:https://doi.org/10.1385/1592590543
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245 1 0 |a Stress Response  |b Methods and Protocols /  |c edited by Stephen M. Keyse. 
250 |a 1st ed. 2000. 
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300 |a XVI, 488 p.  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 99 
505 0 |a Detection and Assay of Stress-Induced Damage -- Identifying and Counting Protein Modifications Triggered by Nitrosative Stress -- Determination of Carbonyl Groups in Oxidized Proteins -- Quantitation of 4-Hydroxynonenal Protein Adducts -- Detection of Oxidative Stress in Lymphocytes Using Dichlorodihydrofluorescein Diacetate -- The Measurement of Protein Degradation in Response to Oxidative Stress -- The Activation of Signal Transduction by Cellular Stress -- Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress -- Detection and Activation of Stress-Responsive Tyrosine Kinases -- Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells -- Expression and Assay of Recombinant ATM -- Detection and Purification of a Multiprotein Kinase Complex from Mammalian Cells -- Methods to Assay Stress-Activated Protein Kinases -- Monitoring the Activation of Stress-Activated Protein Kinases Using GAL4 Fusion Transactivators -- Use of Kinase Inhibitors to Dissect Signaling Pathways -- The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation -- Peptide Assay of Protein Kinases and Use of Variant Peptides to Determine Recognition Motifs -- The Analysis of Stress-Induced Gene Expression -- Assaying NF-KB and AP-1 DNA-Binding and Transcriptional Activity -- Analysis of the Mammalian Heat-Shock Response -- Approaches to Define the Involvement of Reactive Oxygen Species and Iron in Ultraviolet-A Inducible Gene Expression -- The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression -- Sage: The Serial Analysis of Gene Expression -- Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol -- UVB-Regulated Gene Expression in Human Keratinocytes -- Analysis of Stress Protein Function -- Heme Oxygenase Activity Current Methods and Applications -- Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches -- Analysis of Chaperone Properties of Small Hsp’ -- Analysis of Small Hsp Phosphorylation -- Analysis of Multisite Phosphorylation of the p53 Tumor-Suppressor Protein byTryptic Phosphopeptide Mappin -- Development of Physiological Models to Study Stress Protein Responses. 
650 0 |a Cell biology. 
650 1 4 |a Cell Biology. 
700 1 |a Keyse, Stephen M.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1385/1592590543