Stress Response Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Keyse, Stephen M. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2000.
Edición:1st ed. 2000.
Colección:Methods in Molecular Biology, 99
Materias:
Acceso en línea:https://doi.org/10.1385/1592590543
Tabla de Contenidos:
  • Detection and Assay of Stress-Induced Damage
  • Identifying and Counting Protein Modifications Triggered by Nitrosative Stress
  • Determination of Carbonyl Groups in Oxidized Proteins
  • Quantitation of 4-Hydroxynonenal Protein Adducts
  • Detection of Oxidative Stress in Lymphocytes Using Dichlorodihydrofluorescein Diacetate
  • The Measurement of Protein Degradation in Response to Oxidative Stress
  • The Activation of Signal Transduction by Cellular Stress
  • Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress
  • Detection and Activation of Stress-Responsive Tyrosine Kinases
  • Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
  • Expression and Assay of Recombinant ATM
  • Detection and Purification of a Multiprotein Kinase Complex from Mammalian Cells
  • Methods to Assay Stress-Activated Protein Kinases
  • Monitoring the Activation of Stress-Activated Protein Kinases Using GAL4 Fusion Transactivators
  • Use of Kinase Inhibitors to Dissect Signaling Pathways
  • The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation
  • Peptide Assay of Protein Kinases and Use of Variant Peptides to Determine Recognition Motifs
  • The Analysis of Stress-Induced Gene Expression
  • Assaying NF-KB and AP-1 DNA-Binding and Transcriptional Activity
  • Analysis of the Mammalian Heat-Shock Response
  • Approaches to Define the Involvement of Reactive Oxygen Species and Iron in Ultraviolet-A Inducible Gene Expression
  • The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression
  • Sage: The Serial Analysis of Gene Expression
  • Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol
  • UVB-Regulated Gene Expression in Human Keratinocytes
  • Analysis of Stress Protein Function
  • Heme Oxygenase Activity Current Methods and Applications
  • Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches
  • Analysis of Chaperone Properties of Small Hsp’
  • Analysis of Small Hsp Phosphorylation
  • Analysis of Multisite Phosphorylation of the p53 Tumor-Suppressor Protein byTryptic Phosphopeptide Mappin
  • Development of Physiological Models to Study Stress Protein Responses.