Steroid Receptor Methods Protocols and Assays /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Lieberman, Benjamin A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2001.
Edición:1st ed. 2001.
Colección:Methods in Molecular Biology, 176
Materias:
Acceso en línea:https://doi.org/10.1385/1592591159
Tabla de Contenidos:
  • Bioinformatics
  • Bioinformatics of Nuclear Receptors
  • Phylogenetic Inference and Parsimony Analysis
  • Purification Protocols
  • Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems
  • High-Yield Purification of Functionally Active Glucocorticoid Receptor
  • Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor
  • Large Scale Production of Nuclear Receptor Ligand-Binding Domains
  • Physical Structure of Nuclear Receptor-DNA Complexes
  • Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display
  • Identification of Nuclear Hormone Receptor Homologs by Screening Libraries with Highly Degenerate Oligonucleotide Probes
  • Steroid Hormone-Binding Assays
  • Use of [99mTc]Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors
  • Steroid Hormone Metabolites and Hormone Binding Assays
  • Vitamin D3 Analog Screening
  • Protein Interaction Assays
  • Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Subcellular Trafficking of Steroid Receptors
  • Knockout Mice and Steroid Receptor Research
  • Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors
  • Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex
  • Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System
  • Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay
  • Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Transcription In Vivo
  • Cotransfection Assays and Steroid Receptor Biology
  • Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System
  • Cancer Research and Drug Discovery
  • Solid Tumor Cancer Markers and Applications to Steroid Hormone Research
  • Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-7 Focus Assay
  • Combinatorial Chemistry in Steroid Receptor Drug Discovery
  • Identification of Nuclear Receptor Interacting Proteins Using Yeast Two- Hybrid Technology.