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04277nam a22003375i 4500 |
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978-1-59259-177-0 |
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20191022002145.0 |
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cr nn 008mamaa |
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100301s2002 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592591770
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| 024 |
7 |
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|a 10.1385/1592591779
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a PCR Cloning Protocols
|c edited by Bing-Yuan Chen, Harry W. Janes.
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| 250 |
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|a 2nd ed. 2002.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2002.
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| 300 |
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|a XIV, 439 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 192
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| 505 |
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|a Performing and Optimizing PCR -- Polymerase Chain Reaction -- Computer Programs for PCR Primer Design and Analysis -- Single-Step PCR Optimization Using Touchdown and Stepdown PCR Programming -- XL PCR Amplification of Long Targets from Genomic DNA -- Coupled One-Step Reverse Transcription and Polymerase Chain Reaction Procedure for Cloning Large cDNA Fragments -- Long Distance Reverse-Transcription PCR -- Increasing PCR Sensitivity for Amplification from Paraffin-Embedded Tissues -- GC-Rich Template Amplification by Inverse PCR -- PCR Procedure for the Isolation of Trinucleotide Repeats -- Methylation-Specific PCR -- Direct Cloning of Full-Length Cell Differentially Expressed Genes by Multiple Rounds of Subtractive Hybridization Based on Long-Distance PCR and Magnetic Beads -- Cloning PCR Products -- Cloning PCR Products -- Using T4 DNA Polymerase to Generate Clonable PCR Products -- Enzyme-Free Cloning of PCR Products and Fusion Protein Expression -- Directional Restriction Site-Free Insertion of PCR Products into Vectors -- Autosticky PCR -- A Rapid and Simple Procedure for Direct Cloning of PCR Products into Baculoviruses -- Mutagenesis and Recombination -- PCR Approaches to DNA Mutagenesis and Recombination -- In-Frame Cloning of Synthetic Genes Using PCR Inserts -- Megaprimer PCR -- PCR-Mediated Recombination -- PCR Method for Generating Multiple Mutations at Adjacent Sites -- A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing Repeat Expansions into CAG-Repeat Containing Genes -- PCR Screening in Signature-Tagged Mutagenesis of Essential Genes -- Staggered Extension Process (StEP) In Vitro Recombination -- Random Mutagenesis by Whole-Plasmid PCR Amplification -- Cloning Unknown Neighboring DNA -- PCR-Based Strategies to Clone Unknown DNA Regions from Known Foreign Integrants -- Long Distance Vectorette PCR (LDV PCR) -- Nonspecific, Nested Suppression PCR Method for Isolation of Unknown Flanking DNA (“Cold-Start Method”) -- Inverse PCR -- 31 Inverse PCR -- Gene Cloning and Expression Profiling by Rapid Amplification of Gene Inserts with Universal Vector Primers -- The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions by Inverse PCR -- Rapid Amplification of Genomic DNA Sequences Tagged by Insertional Mutagenesis -- Isolation of Large-Terminal Sequences of BAC Inserts Based on Double-Restriction-Enzyme Digestion Followed by Anchored PCR -- A #x201C;Step Down#x201D; PCR-Based Technique for Walking Into and the Subsequent Direct-Sequence Analysis of Flanking Genomic DNA -- Library Construction and Screening -- Use of PCR in Library Screening -- Cloning of Homologous Genes by Gene-Capture PCR -- Rapid and Nonradioactive Screening of Recombinant Libraries by PCR -- Rapid cDNA Cloning by PCR Screening (RC-PCR) -- Generation and PCR Screening of Bacteriophage ? Sublibraries Enriched for Rare Clones (the “Sublibrary Method ”) -- PCR-Based Screening for Bacterial Artificial Chromosome Libraries -- A 384-Well Microtiter-Plate-Based Template Preparation and Sequencing Method -- A Microtiter-Plate-Based High Throughput PCR Product Purification Method.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Science, Humanities and Social Sciences, multidisciplinary.
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| 700 |
1 |
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|a Chen, Bing-Yuan.
|e editor.
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| 700 |
1 |
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|a Janes, Harry W.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
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|u https://doi.org/10.1385/1592591779
|