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04507nam a22002895i 4500 |
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978-1-59259-247-0 |
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20191026172307.0 |
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cr nn 008mamaa |
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100301s1999 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592592470
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| 024 |
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|a 10.1385/1592592473
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Vascular Disease
|b Molecular Biology and Gene Transfer Protocols /
|c edited by Andrew H. Baker.
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| 250 |
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|a 1st ed. 1999.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1999.
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| 300 |
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|a XV, 441 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Medicine,
|v 30
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| 505 |
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|a Genetics in Vascular Disease -- Detection of Mutations and DNA Polymorphisms in Genes Involved in Cardiovascular Diseases by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism Analysis -- Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis -- Radiation Hybrid (RH) Mapping of Human Smooth Muscle-Restricted Genes -- Isolation of Genes Expressed in Vasular Tissue -- Efficient Extraction of RNA from Vascular Tissue -- Preparation of cDNA Libraries from Vascular Cells -- Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells -- Cloning Full-Length cDNAs from Vascular Tissues and Cells by Rapid Amplification of cDNA Ends (RACE) and RT-PCR -- Use of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic Plaques -- The Use of Differential mRNA Display (DDRT-PCR) to Identify Genes Differentially Expressed in Normal and Diseased Vascular Cells -- Identification of Novel Protein Kinases in Vascular Cells -- Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular Cells -- Primer Extension Analysis to Map Transcription Start Sites of Vascular Genes -- Use of Liposome-Mediated DNA Transfection to Determine Promoter Activity in Smooth Muscle Cells -- Nuclear Run-On Assay to Study Gene Transcription in Vascular Cells -- Electromobility Shift Analysis (EMSA) Applied to the Study of NF-kappa B Binding Interactions in Vascular Smooth Muscle Cells -- Molecular Analysis of MRNA Expression in Vascular Cells -- Measurement of Gene Expression in the Vascular Wall by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis -- Northern Blot Analysis to Quantify Gene Expression in Vascular Diseases -- Localization of Gene Expression in Human Atherosclerotic Lesions by In SituHybridization -- RNase Protection Assays for Quantitation of Gene Expression in Vascular Tissue -- Molecular Methods to Study Apoptosis and Phenotypic Changes in Vascular Cells and Tissus -- Detection of Apoptosis in Atherosclerosis and Restenosis by Terminal dUTP Nick-End Labeling (TUNEL) -- In Vitro Detection of Apoptosis in Isolated Vascular Cells -- Molecular Assessment of the Phenotypic Changes Associated with Smooth Muscle Cells Using Two-Dimension Electrophoresis and Microsequence Analysis -- In Vitro Methods to Express Foreign Genes in Vasular Cells -- Simple Methods for Preparing Recombinant Adenoviruses for High-Efficiency Transduction of Vascular Cells -- Generation of Recombinant Adeno-Associated Viruses for Delivery of Genes into Vascular Cells -- Hemagglutinating Virus of Japan Liposome-Mediated Gene Delivery to Vascular Cells -- High-Efficiency and Low-Toxicity Adenovirus-Assisted Endothelial Transfection -- Gene Transfer in Vascular Cells Using an Engineered Na-H Exchanger (NHE1) as a Selectable Marker -- Use of Retroviruses to Express Exogenous Genes in Vascular Smooth Muscle Cells -- Embryonal Stem (ES) Cell-Derived Macrophages -- Delivery of Recombinant Adenoviruses to Human Saphenous Vein -- In Vivo Vascular Gene Transfer Protocols -- An In Vivo Angiogenesis Assay to Study Positive and Negative Regulators of Neovascularization -- Efficient Liposome-Mediated Gene Transfer to Rabbit Carotid Arteries In Vivo -- Gene Delivery to Rabbit Arteries Using the Collar Model -- Delivery of Antisense Oligonucleotides to the Vascular Wall -- Local Gene Delivery of Recombinant Adenoviruses to the Rat Carotid Artery In Vivo.
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| 650 |
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|a Vascular surgery.
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| 650 |
1 |
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|a Vascular Surgery.
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| 700 |
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|a Baker, Andrew H.
|e editor.
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| 710 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592592473
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