|
|
|
|
| LEADER |
03432nam a22002895i 4500 |
| 001 |
978-1-59259-255-5 |
| 005 |
20190617231755.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100301s1998 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781592592555
|
| 024 |
7 |
|
|a 10.1385/0896034917
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Phospholipid Signaling Protocols
|c edited by Ian Bird.
|
| 250 |
|
|
|a 1st ed. 1998.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
|
| 300 |
|
|
|a XIII, 380 p. 22 illus.
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 105
|
| 505 |
0 |
|
|a Monitoring of Activation of Phospholipid-Derived Cell Signaling Pathways -- Phosphoinositidase C Activation Assay I -- Phosphoinositidase C Activation Assay II -- Phosphoinositidase C Activation Assay III -- Measurement of Phosphoinositols and Phosphoinositides Using Radio High-Performance Liquid Chromatography Flow Detection -- Preparation of [3H]Phosphoinositol Standards and Conversion of [3H]Phosphoinositides to [3H]Phosphoinositols -- Inositol 1,4,5-Trisphosphate Mass Assay -- Measurement of Cellular Diacylglycerol Content -- Phosphatidylinositol 4-Kinases -- Phosphatidylinositol(3,4,5)Trisphosphate (Ptdins(3,4,5)P3) Mass Measurement Using a Radioligand Displacement Assay -- Detection of Phosphatidylinositol-4-Phosphate 5-Kinase Activity Using Thin-Layer Chromatography -- Determination of Phospholipase C-or Phospholipase D-Catalyzed Phosphatidylcholine Hydrolysis -- Measurement of Phospholipase D Activity -- Monitoring of Phospholipase A2 Activation in Cultured Cells Using Tritiated Arachidonic Acid -- Assay of Cellular Diacylglycerol and Monoacylglycerol Lipases -- Isotopic Efflux Studies as Indices of Phospholipase Activation -- Extraction and Measurements of Prostanoids and Leukotrienes by Radioimmunoassays -- Measurements of Prostanoids, Leukotrienes, and Isoprostanes by Enzyme Immunoassays -- Measurements of Arachidonic Acid Metabolites Derived from the Lipoxygenase Pathways by High-Pressure Liquid Chromatography -- Measurement of Sphingomyelin and Ceramide Cellular Levels After Sphingomyelinase-Mediated Sphingomyelin Hydrolysis -- Sphingomyelin and Ceramide Mass Assay -- Sphingosine Kinase -- Analytical Methods and Steps to Sample Preparation for Determination of Molecular Species of Fatty Acids -- HPLC Analytical Methods for the Separation of Molecular Species of Fatty Acids in Diacylglycerol and Cellular Phospholipids -- Analysis of Molecular Species of Cellular Sphingomyelins and Ceramides -- General Methods for Monitoring Changes in Levels of Key Signaling Pathway Proteins and Associated mRNA -- Solubilization and Assay of Cellular and Tissue Protein -- Western Immunoblot Analysis -- Immunohistochemistry and Immunocytochemistry -- Extraction of Cellular and Tissue RNA -- Size Separation and Quantification of mRNA by Northern Analysis -- Preparation of Single-Stranded Antisense cDNA Probes by Asymmetric PCR -- Optimization of a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Mass Assay for Low-Abundance mRNA.
|
| 650 |
|
0 |
|a Biochemistry.
|
| 650 |
1 |
4 |
|a Biochemistry, general.
|
| 700 |
1 |
|
|a Bird, Ian.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896034917
|