Phospholipid Signaling Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1998.
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| Edición: | 1st ed. 1998. |
| Colección: | Methods in Molecular Biology,
105 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896034917 |
Tabla de Contenidos:
- Monitoring of Activation of Phospholipid-Derived Cell Signaling Pathways
- Phosphoinositidase C Activation Assay I
- Phosphoinositidase C Activation Assay II
- Phosphoinositidase C Activation Assay III
- Measurement of Phosphoinositols and Phosphoinositides Using Radio High-Performance Liquid Chromatography Flow Detection
- Preparation of [3H]Phosphoinositol Standards and Conversion of [3H]Phosphoinositides to [3H]Phosphoinositols
- Inositol 1,4,5-Trisphosphate Mass Assay
- Measurement of Cellular Diacylglycerol Content
- Phosphatidylinositol 4-Kinases
- Phosphatidylinositol(3,4,5)Trisphosphate (Ptdins(3,4,5)P3) Mass Measurement Using a Radioligand Displacement Assay
- Detection of Phosphatidylinositol-4-Phosphate 5-Kinase Activity Using Thin-Layer Chromatography
- Determination of Phospholipase C-or Phospholipase D-Catalyzed Phosphatidylcholine Hydrolysis
- Measurement of Phospholipase D Activity
- Monitoring of Phospholipase A2 Activation in Cultured Cells Using Tritiated Arachidonic Acid
- Assay of Cellular Diacylglycerol and Monoacylglycerol Lipases
- Isotopic Efflux Studies as Indices of Phospholipase Activation
- Extraction and Measurements of Prostanoids and Leukotrienes by Radioimmunoassays
- Measurements of Prostanoids, Leukotrienes, and Isoprostanes by Enzyme Immunoassays
- Measurements of Arachidonic Acid Metabolites Derived from the Lipoxygenase Pathways by High-Pressure Liquid Chromatography
- Measurement of Sphingomyelin and Ceramide Cellular Levels After Sphingomyelinase-Mediated Sphingomyelin Hydrolysis
- Sphingomyelin and Ceramide Mass Assay
- Sphingosine Kinase
- Analytical Methods and Steps to Sample Preparation for Determination of Molecular Species of Fatty Acids
- HPLC Analytical Methods for the Separation of Molecular Species of Fatty Acids in Diacylglycerol and Cellular Phospholipids
- Analysis of Molecular Species of Cellular Sphingomyelins and Ceramides
- General Methods for Monitoring Changes in Levels of Key Signaling Pathway Proteins and Associated mRNA
- Solubilization and Assay of Cellular and Tissue Protein
- Western Immunoblot Analysis
- Immunohistochemistry and Immunocytochemistry
- Extraction of Cellular and Tissue RNA
- Size Separation and Quantification of mRNA by Northern Analysis
- Preparation of Single-Stranded Antisense cDNA Probes by Asymmetric PCR
- Optimization of a Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Mass Assay for Low-Abundance mRNA.